Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,372,254	G→A	G107G (GGC→GGT)	nanT ←	sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,372,254	0	G	A	100.0%	113.9 / NA	36	G107G (GGC→GGT)	nanT	sialic acid transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/14); total (22/14)

AAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACC  >  NC_000913/3372130‑3372369

aaaaaCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGcc                                                                                                       >  1:312582/1‑139 (MQ=255)

         tGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAg                                                                                              >  1:161171/1‑139 (MQ=255)

              ttGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTcc                                                                                         <  1:591238/139‑1 (MQ=255)

                   aCGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGcc                                                                                    <  2:124540/139‑1 (MQ=255)

                                      cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTgg                                                                 >  1:387898/1‑139 (MQ=255)

                                           aaTGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAcc                                                            >  2:945014/1‑139 (MQ=255)

                                                cATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGc                                                       >  1:344816/1‑139 (MQ=255)

                                                cATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGc                                                       >  2:751694/1‑139 (MQ=255)

                                                         cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTg                                                                  <  2:145807/119‑1 (MQ=255)

                                                         cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTg                                                                  >  1:145807/1‑119 (MQ=255)

                                                          gcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                     <  2:637893/115‑1 (MQ=255)

                                                          gcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                     >  1:637893/1‑115 (MQ=255)

                                                               aaCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTa                                        >  1:130405/1‑139 (MQ=255)

                                                                aCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAg                                       >  1:306785/1‑139 (MQ=255)

                                                                  cGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCg                                     <  2:306785/139‑1 (MQ=255)

                                                                   gTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCgg                                    >  1:956937/1‑139 (MQ=255)

                                                                           ccGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACg                                                                        >  1:280718/1‑95 (MQ=255)

                                                                           ccGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACg                                                                        <  2:280718/95‑1 (MQ=255)

                                                                           ccGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCAt                            >  1:1160668/1‑139 (MQ=255)

                                                                                  ccccATGCCGATGACCAGACAAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGcc                                                                                    >  2:1316236/1‑76 (MQ=255)

                                                                                  ccccATGCCGATGACCAGACAAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGcc                                                                                    <  1:1316236/76‑1 (MQ=255)

                                                                                         ccGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGCAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGGCACCCATAGCGCCGAGCATCa              >  2:379148/1‑139 (MQ=255)

                                                                                          cGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAg             <  2:130405/139‑1 (MQ=255)

                                                                                            aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGc                                                       >  2:493661/1‑95 (MQ=255)

                                                                                            aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGc                                                       <  1:493661/95‑1 (MQ=255)

                                                                                             tGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaa                                                                               <  1:1242722/70‑1 (MQ=255)

                                                                                             tGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaa                                                                               >  2:1242722/1‑70 (MQ=255)

                                                                                                   gACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAg                                                                                              <  2:1330151/49‑1 (MQ=255)

                                                                                                   gACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAg                                                                                              >  1:1330151/1‑49 (MQ=255)

                                                                                                   gACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGaa    >  1:735055/1‑139 (MQ=255)

                                                                                                        gCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTg                                                                  >  2:278092/1‑72 (MQ=255)

                                                                                                        gCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTg                                                                  <  1:278092/72‑1 (MQ=255)

                                                                                                         cGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaag                                                                              >  1:596029/1‑59 (MQ=255)

                                                                                                         cGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaag                                                                              <  2:596029/59‑1 (MQ=255)

                                                                                                                  tGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAAcc  >  2:340562/1‑126 (MQ=255)

                                                                                                                  tGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAAcc  <  1:340562/126‑1 (MQ=255)

AAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACC  >  NC_000913/3372130‑3372369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A