Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,372,254	G→A	G107G (GGC→GGT)	nanT ←	sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,372,254	0	G	A	100.0%	139.8 / NA	44	G107G (GGC→GGT)	nanT	sialic acid transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/21); total (23/21)

CTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGC  >  NC_000913/3372121‑3372388

cTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTgg                                                                                                                                     <  1:409065/139‑1 (MQ=255)

  gAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGgcg                                                                                                                                   >  1:866054/1‑139 (MQ=255)

    aaTCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCa                                                                                                                                 >  2:811370/1‑139 (MQ=255)

       cAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAg                                                                                                                              <  2:783013/139‑1 (MQ=255)

                       ttGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTcc                                                                                                              <  1:554268/139‑1 (MQ=255)

                              gCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCga                                                                                                       >  2:195203/1‑139 (MQ=255)

                                 gATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaa                                                                                                    <  2:700820/139‑1 (MQ=255)

                                  aTGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaag                                                                                                   >  2:428102/1‑139 (MQ=255)

                                    gTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                <  2:566580/108‑1 (MQ=255)

                                    gTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                >  1:566580/1‑108 (MQ=255)

                                    gTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagag                                                                                                 >  2:238531/1‑139 (MQ=255)

                                            cAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGc                                                                                         >  2:488535/1‑139 (MQ=255)

                                                   cAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGAc                                                                                <  1:238531/139‑1 (MQ=255)

                                                         cATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGc                                                                          >  1:657657/1‑139 (MQ=255)

                                                            aGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAg                                                                       >  2:702426/1‑139 (MQ=255)

                                                            aGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAg                                                                       >  2:677097/1‑139 (MQ=255)

                                                              gTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAgac                                                                     <  2:866054/139‑1 (MQ=255)

                                                                  cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGacgacg                                                                 >  1:90892/1‑139 (MQ=255)

                                                                  cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGacgacg                                                                 <  1:811370/139‑1 (MQ=255)

                                                                  cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGacgacg                                                                 >  1:488985/1‑139 (MQ=255)

                                                                             tATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCg                                                        <  2:133243/137‑1 (MQ=255)

                                                                             tATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCg                                                        >  1:133243/1‑137 (MQ=255)

                                                                             tATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGt                                                      <  1:677097/139‑1 (MQ=255)

                                                                                  aCCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                >  1:845850/1‑62 (MQ=255)

                                                                                  aCCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                <  2:845850/62‑1 (MQ=255)

                                                                                    ccGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCAt                                               >  2:24127/1‑139 (MQ=255)

                                                                                     cGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATa                                              <  2:488985/139‑1 (MQ=255)

                                                                                           ccccATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCg                                        <  2:657657/139‑1 (MQ=255)

                                                                                              cATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGTCGCaa                                                                                                                                >  2:974033/1‑50 (MQ=255)

                                                                                              cATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                <  1:974033/50‑1 (MQ=255)

                                                                                                     aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAg                                                          <  2:75442/111‑1 (MQ=255)

                                                                                                     aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAg                                                          >  1:75442/1‑111 (MQ=255)

                                                                                                     aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgc                              >  2:917699/1‑139 (MQ=255)

                                                                                                          cAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGccgccg                         <  2:90892/139‑1 (MQ=255)

                                                                                                             aCGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAAc                      >  2:594781/1‑139 (MQ=255)

                                                                                                             aCGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAAc                      >  2:289628/1‑139 (MQ=255)

                                                                                                                   gATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCga                <  1:488535/139‑1 (MQ=255)

                                                                                                                   gATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCga                <  1:428102/139‑1 (MQ=255)

                                                                                                                           tGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATaaa        <  2:416388/139‑1 (MQ=255)

                                                                                                                              taaTGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAGGAGAATGATCCTGGTGTGACCATTGCCAGCCGAGGCCCGTAGCGGTCACCCATAGCGGCGGGCATCAGGCGGCAGAACCCGCGAGATATACAg       >  2:299418/3‑139 (MQ=255)

                                                                                                                               gATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTc                                                     <  1:127802/90‑1 (MQ=255)

                                                                                                                               gATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTc                                                     >  2:127802/1‑90 (MQ=255)

                                                                                                                                 tGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGc  <  1:824663/139‑1 (MQ=255)

                                                                                                                                 tGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGc  >  2:824663/1‑139 (MQ=255)

CTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGC  >  NC_000913/3372121‑3372388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A