Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
477,435 |
T→C |
T395A (ACT→GCT) |
ylaB ← |
putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 477,435 | 0 | T | C | 100.0%
| 137.7
/ NA
| 40 | T395A (ACT→GCT) | ylaB | putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/20); total (20/20) |
ATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCG > NC_000913/477310‑477542
|
aTCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGcgcg > 1:651570/1‑139 (MQ=255)
ccTGTAAATAACTTAAACTTGAATACCCCTTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt < 2:497351/139‑1 (MQ=255)
gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctg > 2:129797/1‑139 (MQ=255)
gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctg > 2:607389/1‑139 (MQ=255)
tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt > 2:653094/1‑139 (MQ=255)
tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt > 2:315163/1‑139 (MQ=255)
tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt > 2:533368/1‑139 (MQ=255)
tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt > 1:698279/1‑139 (MQ=255)
tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt > 1:358897/1‑139 (MQ=255)
aaaTAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgtc < 2:651570/139‑1 (MQ=255)
aaaTAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgtc < 1:533368/139‑1 (MQ=255)
cTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCt < 2:362796/139‑1 (MQ=255)
aaTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATa < 2:453139/139‑1 (MQ=255)
aTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGCTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAg > 1:185378/1‑139 (MQ=255)
cccGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGtgat > 1:432324/1‑139 (MQ=255)
tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTGAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGa < 1:515689/128‑1 (MQ=255)
tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGa > 2:515689/1‑128 (MQ=255)
tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGtgattgat > 2:410687/1‑139 (MQ=255)
aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt < 1:588577/108‑1 (MQ=255)
aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt > 2:588577/1‑108 (MQ=255)
aacgcatcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTGCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGt > 2:496142/5‑139 (MQ=255)
tcaAGATAAGTTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCATCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGa < 2:164473/117‑1 (MQ=255)
tcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGa > 1:164473/1‑117 (MQ=255)
caAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt < 1:444933/97‑1 (MQ=255)
caAGATAAATTTCATGGCCCGCCTCCCGGAAGCGAGAAATTATCGAGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt > 2:444933/1‑97 (MQ=255)
aaTTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGc < 2:432324/139‑1 (MQ=255)
tGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGAttt > 1:270676/1‑139 (MQ=255)
cccGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGt < 1:374162/139‑1 (MQ=255)
gCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGCTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACg < 2:185378/139‑1 (MQ=255)
gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTTCGAGGTGAGCACggg < 1:653094/139‑1 (MQ=255)
gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACggg < 1:129797/139‑1 (MQ=255)
tggtctcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGGTCAAGCGGGAGCT‑TCAGAGGTGTAACCTGGTAGTGATTGATTATGTCACGCAGCAACTGCAGGATTTTTACCGAGGTGAGCACGGGCAATTCGAGAt < 2:28189/133‑1 (MQ=255)
gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGtt > 2:103681/1‑40 (MQ=255)
gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGtt < 1:103681/40‑1 (MQ=255)
gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTg‑g > 1:656346/1‑58 (MQ=255)
gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTg‑g < 2:656346/58‑1 (MQ=255)
cgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGAt < 2:259736/139‑1 (MQ=255)
cTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCg < 1:438042/139‑1 (MQ=255)
cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGAtt > 1:685234/1‑46 (MQ=255)
cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTG‑GGAtt < 2:685234/46‑1 (MQ=255)
|
ATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTG‑GGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCG > NC_000913/477310‑477542
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
