Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	991,794	C→T	I391I (ATC→ATT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	991,794	0	C	T	100.0%	111.9 / NA	36	I391I (ATC→ATT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/15); total (21/15)

ACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTC  >  NC_000913/991660‑991929

aCCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTcaca                                                                                                                                     <  2:452564/139‑1 (MQ=255)

aCCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTcaca                                                                                                                                     >  1:339327/1‑139 (MQ=255)

    tGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCt                                                                                                                                 >  1:661532/1‑139 (MQ=255)

     gcgcgcATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTg                                                                                                                                >  1:435496/1‑139 (MQ=255)

                    tGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAAc                                                                                                                 >  2:337630/1‑139 (MQ=255)

                            aCGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGaa                                                                                                         >  1:577240/1‑139 (MQ=255)

                                   cccGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATg                                                                                                  <  2:435496/139‑1 (MQ=255)

                                                  gATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAg                                                                                   <  1:337630/139‑1 (MQ=255)

                                                    tCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCg                                                                                 >  1:103021/1‑139 (MQ=255)

                                                     ccGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGt                                                                                <  2:339327/139‑1 (MQ=255)

                                                        cccGGATATGGTCATTGAGATGAACAACTTCTACACCCTGGCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCat                                                                             <  2:561585/139‑1 (MQ=255)

                                                        cccGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCat                                                                             >  2:653840/1‑139 (MQ=255)

                                                                        gagaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGa                                                             <  2:208693/139‑1 (MQ=255)

                                                                             gAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCtgt                                                        >  2:106436/1‑139 (MQ=255)

                                                                               acaacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTga                                                      >  2:244841/1‑139 (MQ=255)

                                                                                     tCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACtt                                                >  1:553068/1‑139 (MQ=255)

                                                                                           cccTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCa                                          <  1:653840/139‑1 (MQ=255)

                                                                                              tGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGc                                       >  1:543629/1‑139 (MQ=255)

                                                                                              tGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGc                                       >  1:680594/1‑139 (MQ=255)

                                                                                              tGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGc                                       >  1:97930/1‑139 (MQ=255)

                                                                                              tGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGc                                       >  2:755319/1‑139 (MQ=255)

                                                                                                aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATg                                                                                                  <  1:545501/78‑1 (MQ=255)

                                                                                                aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATg                                                                                                  >  2:545501/1‑78 (MQ=255)

                                                                                                aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCa                                                                 >  1:290253/1‑111 (MQ=255)

                                                                                                aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCa                                                                 <  2:290253/111‑1 (MQ=255)

                                                                                                aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa                                     >  1:200031/1‑139 (MQ=255)

                                                                                                      tACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGc                                                                                  >  2:524585/1‑88 (MQ=255)

                                                                                                      tACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGc                                                                                  <  1:524585/88‑1 (MQ=255)

                                                                                                      tACGAGAAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGACGATGGacg                               >  2:454439/1‑137 (MQ=255)

                                                                                                           gaAGGGCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCg                          <  2:103021/139‑1 (MQ=255)

                                                                                                              gggCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGa                                                                                                          >  2:204290/1‑56 (MQ=255)

                                                                                                              gggCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGa                                                                                                          <  1:204290/56‑1 (MQ=255)

                                                                                                               ggCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGt                                                                                <  1:608231/81‑1 (MQ=255)

                                                                                                               ggCGCGGAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGt                                                                                >  2:608231/1‑81 (MQ=255)

                                                                                                                     gAAGTGATTCGCATGATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCg                <  1:275275/139‑1 (MQ=255)

                                                                                                                                   gATTCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTc  <  2:661532/139‑1 (MQ=255)

ACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTC  >  NC_000913/991660‑991929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A