Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,225,089 |
T→C |
T91T (ACA→ACG) |
minD ← |
inhibitor of FtsZ ring polymerization, chromosome‑membrane tethering protein, membrane ATPase of the MinCDEE system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,225,089 | 0 | T | C | 100.0%
| 89.2
/ NA
| 26 | T91T (ACA→ACG) | minD | inhibitor of FtsZ ring polymerization, chromosome‑membrane tethering protein, membrane ATPase of the MinCDEE system |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/13); total (13/13) |
TGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAAT > NC_000913/1224966‑1225217
|
tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGc > 1:58245/1‑139 (MQ=255)
gCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCa < 2:710140/139‑1 (MQ=255)
cATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGa < 2:716108/139‑1 (MQ=255)
ttAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAat > 1:178280/1‑139 (MQ=255)
aCCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAgaga > 1:28202/1‑139 (MQ=255)
aaTCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTa < 2:28202/139‑1 (MQ=255)
ccTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCt < 2:321764/139‑1 (MQ=255)
ccTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCt < 2:178280/139‑1 (MQ=255)
aaTTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTa > 1:379867/1‑139 (MQ=255)
aaaTCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTg > 1:362302/1‑139 (MQ=255)
atcaAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTg > 1:259046/1‑139 (MQ=255)
caAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGAc < 2:362302/139‑1 (MQ=255)
aaGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACg < 2:259046/139‑1 (MQ=255)
gAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGaa > 1:496541/1‑139 (MQ=255)
tGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGt > 1:597799/1‑138 (MQ=255)
tGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGt < 2:597799/138‑1 (MQ=255)
gTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGAt < 2:525163/49‑1 (MQ=255)
gTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTcac < 1:81421/139‑1 (MQ=255)
gTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTcac < 2:379867/139‑1 (MQ=255)
gTGAGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTcac < 2:770951/139‑1 (MQ=255)
gTGAGGGCATCTTTATAGCGCGTTTGCGATGCCGGCAGAATATAGAGAt > 1:525163/1‑49 (MQ=255)
aGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATTTAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAAc > 2:82502/1‑139 (MQ=255)
aGGGCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAAc > 2:86708/1‑139 (MQ=255)
ggCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGAc > 1:754787/1‑123 (MQ=255)
ggCATCTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGAc < 2:754787/123‑1 (MQ=255)
cTTTATCGCGCGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAAt > 2:168718/1‑139 (MQ=255)
|
TGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAAT > NC_000913/1224966‑1225217
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
