Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,539,230 |
G→A |
G118G (GGC→GGT) |
cysM ← |
cysteine synthase B (O‑acetylserine sulfhydrolase B) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,539,230 | 0 | G | A | 100.0%
| 71.8
/ NA
| 24 | G118G (GGC→GGT) | cysM | cysteine synthase B (O‑acetylserine sulfhydrolase B) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/11); total (13/11) |
GTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATG > NC_000913/2539123‑2539365
|
gtggtggtgTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCg < 2:755190/139‑1 (MQ=255)
gtggtggtgTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCg < 1:726879/139‑1 (MQ=255)
cgcATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGc > 1:125854/1‑139 (MQ=255)
aTCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACgg > 2:711828/1‑139 (MQ=255)
gAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTc < 2:589541/139‑1 (MQ=255)
cTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAAt < 1:731408/90‑1 (MQ=255)
cTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAAt > 2:731408/1‑90 (MQ=255)
aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGa < 1:535839/80‑1 (MQ=255)
aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGa > 2:535839/1‑80 (MQ=255)
aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACg > 2:374867/1‑120 (MQ=255)
aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACg < 1:374867/120‑1 (MQ=255)
aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCAtgttg > 1:614656/1‑139 (MQ=255)
gCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTTATGTTGTCGGGCATCAGCAATTTCATGCGATAGc > 1:267994/1‑139 (MQ=255)
tctcCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGAACCATAAGCAGGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCttt < 2:717719/139‑1 (MQ=255)
gATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCA‑GTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCgg > 1:272828/1‑134 (MQ=255)
gATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCA‑GTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCgg < 2:272828/134‑1 (MQ=255)
cgcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTg > 2:534246/1‑139 (MQ=255)
cgcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTg > 1:184036/1‑139 (MQ=255)
cgcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTg > 1:17972/1‑139 (MQ=255)
cgcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTg > 1:144985/1‑139 (MQ=255)
gcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGc < 2:646201/139‑1 (MQ=255)
cgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGcc < 2:125854/139‑1 (MQ=255)
cTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAgcgc > 1:651136/1‑139 (MQ=255)
cATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATg < 1:534246/139‑1 (MQ=255)
|
GTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATG > NC_000913/2539123‑2539365
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
