Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,682,677 |
T→C |
I23M (ATA→ATG) |
yphG ← |
DUF4380 domain‑containing TPR repeat protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,682,677 | 0 | T | C | 100.0%
| 105.3
/ NA
| 30 | I23M (ATA→ATG) | yphG | DUF4380 domain‑containing TPR repeat protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/19); total (11/19) |
TAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCA > NC_000913/2682546‑2682793
|
tAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGCACATGGGATGCATATCCt < 2:427139/139‑1 (MQ=255)
tCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCg > 1:10989/1‑139 (MQ=255)
ccACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCa < 2:390477/139‑1 (MQ=255)
cacaCCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCAt < 1:750019/139‑1 (MQ=255)
acCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAgg > 1:176847/1‑139 (MQ=255)
tGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGa > 1:76024/1‑139 (MQ=255)
ccAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGAtc < 1:695588/139‑1 (MQ=255)
gctcgctcAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCacac < 2:108131/139‑1 (MQ=255)
tCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGt < 1:527098/139‑1 (MQ=255)
tAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGAtc < 1:224240/87‑1 (MQ=255)
tAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGAtc > 2:224240/1‑87 (MQ=255)
ccGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCca < 2:727068/100‑1 (MQ=255)
ccGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCca > 1:727068/1‑100 (MQ=255)
ccGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAg < 2:400555/139‑1 (MQ=255)
cgcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAat > 1:679635/1‑127 (MQ=255)
cgcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAat < 2:679635/127‑1 (MQ=255)
cgcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACa > 2:157909/1‑139 (MQ=255)
gcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCTGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACAt < 2:101776/139‑1 (MQ=255)
gcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACttt < 2:698475/102‑1 (MQ=255)
gcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACttt > 1:698475/1‑102 (MQ=255)
aTAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAAt < 2:256991/131‑1 (MQ=255)
aTAAACGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAAt > 1:256991/1‑131 (MQ=255)
aaCGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCTACGCGCTCGTGCCACGCTTTTACTGTAGTCATGGTGCCCTCAATTTTAGCAAGACATACGGAATTAAAAGATTtg < 2:176847/139‑1 (MQ=255)
aaCGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTtg < 2:447467/139‑1 (MQ=255)
aCGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTg > 2:275882/1‑88 (MQ=255)
aCGCGATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTg < 1:275882/88‑1 (MQ=255)
gcgATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTgg < 1:323036/139‑1 (MQ=255)
gATTTTCCAGGAACATGGGATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCa < 2:10989/139‑1 (MQ=255)
gggATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGctc < 1:621091/51‑1 (MQ=255)
gggATGCATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGctc > 2:621091/1‑51 (MQ=255)
|
TAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCA > NC_000913/2682546‑2682793
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
