Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,310,291	G→A	S230S (AGC→AGT)	alsA ←	D‑allose ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,310,291	0	G	A	100.0%	104.4 / NA	33	S230S (AGC→AGT)	alsA	D‑allose ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/16); total (17/16)

TCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAG  >  NC_000913/4310157‑4310425

tCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTa                                                                                                                                    >  2:766586/1‑139 (MQ=255)

tCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTa                                                                                                                                    >  2:650171/1‑139 (MQ=255)

  aCGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACt                                                                                                                                  >  2:532967/1‑139 (MQ=255)

        ggTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCg                                                                                                                            >  1:657435/1‑139 (MQ=255)

          tGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCt                                                                                                                          >  1:309832/1‑139 (MQ=255)

          tGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCt                                                                                                                          >  1:659250/1‑139 (MQ=255)

                     aCCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACgctgc                                                                                                               <  1:403626/139‑1 (MQ=255)

                        tCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCtgctgc                                                                                                            <  1:82863/139‑1 (MQ=255)

                          aaaaaCCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTGGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCg                                                                                                          >  2:579315/1‑139 (MQ=255)

                               ccGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCttt                                                                                                     <  1:67068/139‑1 (MQ=255)

                                 gTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATg                                                                                                                               >  1:56149/1‑111 (MQ=255)

                                 gTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATg                                                                                                                               <  2:56149/111‑1 (MQ=255)

                                 gTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtca                                                                                                   <  1:450214/139‑1 (MQ=255)

                                    tCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtcatca                                                                                                <  2:122577/139‑1 (MQ=255)

                                    tCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtcatca                                                                                                >  1:369046/1‑139 (MQ=255)

                                                          tctcCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtcat                                                                                                  <  2:692353/115‑1 (MQ=255)

                                                          tctcCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtcat                                                                                                  >  1:692353/1‑115 (MQ=255)

                                                             ccTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCg                                                                       >  2:196253/1‑139 (MQ=255)

                                                                      cgTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGc                                                              >  2:763183/1‑139 (MQ=255)

                                                                        ttAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCa                                                            >  1:683107/1‑139 (MQ=255)

                                                                               ggTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATg                                                     <  2:120874/139‑1 (MQ=255)

                                                                                 ttttGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCg                                                   <  1:382390/139‑1 (MQ=255)

                                                                                 ttttGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCg                                                   <  1:650171/139‑1 (MQ=255)

                                                                                 ttttGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCg                                                   <  1:161223/139‑1 (MQ=255)

                                                                                   ttGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCgag                                                 <  1:178342/139‑1 (MQ=255)

                                                                                             cgGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGa                                       <  1:651249/139‑1 (MQ=255)

                                                                                                     cATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTa                               <  1:532967/139‑1 (MQ=255)

                                                                                                              gacgATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTa                      >  1:11832/1‑139 (MQ=255)

                                                                                                                cgATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACg                    <  1:766586/139‑1 (MQ=255)

                                                                                                                             tGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTa                      <  2:20695/124‑1 (MQ=255)

                                                                                                                             tGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTa                      >  1:20695/1‑124 (MQ=255)

                                                                                                                             tGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATg       >  2:571149/1‑139 (MQ=255)

                                                                                                                                  caTCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAg  >  1:368384/1‑139 (MQ=255)

TCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAG  >  NC_000913/4310157‑4310425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A