Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,310,291	G→A	S230S (AGC→AGT)	alsA ←	D‑allose ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,310,291	0	G	A	100.0%	107.3 / NA	35	S230S (AGC→AGT)	alsA	D‑allose ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/15); total (20/15)

TCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGAT  >  NC_000913/4310157‑4310422

tCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTa                                                                                                                                 >  1:625470/1‑139 (MQ=255)

 cACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTAc                                                                                                                                >  1:158658/1‑139 (MQ=255)

                       cTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGctgctg                                                                                                          >  1:127033/1‑139 (MQ=255)

                        tCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCtgctgc                                                                                                         <  2:28251/139‑1 (MQ=255)

                                 gTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtca                                                                                                >  2:692416/1‑139 (MQ=255)

                                 gTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtca                                                                                                >  1:637595/1‑139 (MQ=255)

                                    tCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtcatca                                                                                             <  1:527897/139‑1 (MQ=255)

                                              ggTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtcat                                                                                               >  2:523236/1‑127 (MQ=255)

                                              ggTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtcat                                                                                               <  1:523236/127‑1 (MQ=255)

                                                  gCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCg                                                                               >  1:776339/1‑139 (MQ=255)

                                                        aTTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATa                                                                         >  1:157047/1‑139 (MQ=255)

                                                             ccTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACg                                                                                                                <  2:335820/95‑1 (MQ=255)

                                                             ccTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACg                                                                                                                >  1:335820/1‑95 (MQ=255)

                                                                    cgcgTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTcc                                                             >  1:490811/1‑139 (MQ=255)

                                                                      cgTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGc                                                           <  1:210277/139‑1 (MQ=255)

                                                                      cgTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGc                                                           >  1:358808/1‑139 (MQ=255)

                                                                      cgTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGc                                                           >  2:209628/1‑139 (MQ=255)

                                                                      cgTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATCTCCGc                                                           >  1:538974/1‑139 (MQ=255)

                                                                                           cgcgGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtcat                                                                                               >  1:257166/1‑82 (MQ=255)

                                                                                           cgcgGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTtcat                                                                                               <  2:257166/82‑1 (MQ=255)

                                                                                           cgcgGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGAc                                      <  2:127033/139‑1 (MQ=255)

                                                                                           cgcgGCCTACCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGAc                                      >  2:558834/1‑139 (MQ=255)

                                                                                                   aCCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGa                                    >  1:718100/1‑133 (MQ=255)

                                                                                                   aCCATCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGa                                    <  2:718100/133‑1 (MQ=255)

                                                                                                      aTCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTAc                           <  1:85447/139‑1 (MQ=255)

                                                                                                      aTCAGACGGACGATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTAc                           <  2:538974/139‑1 (MQ=255)

                                                                                                          gacggacgATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGa                                    >  1:620813/1‑126 (MQ=255)

                                                                                                          gacggacgATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGa                                    <  2:620813/126‑1 (MQ=255)

                                                                                                          gacggacgATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCctc                       >  1:473506/1‑139 (MQ=255)

                                                                                                              gacgATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTa                   >  2:289569/1‑139 (MQ=255)

                                                                                                                cgATATCGTCATTTGACACATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACg                 <  1:209628/139‑1 (MQ=255)

                                                                                                                               acacATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGAt  <  2:158658/139‑1 (MQ=255)

                                                                                                                               acacATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGAt  <  2:625470/139‑1 (MQ=255)

                                                                                                                               acacATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGAt  >  2:703547/1‑139 (MQ=255)

                                                                                                                               acacATCACTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGAt  <  2:776339/139‑1 (MQ=255)

TCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGGCCTACCATCAGACGGACGATATCGTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGAT  >  NC_000913/4310157‑4310422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A