Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,433,164	C→T	287 (TAG→TAA)	qorB ←	NAD(P)H:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,433,164	0	C	T	100.0%	94.8 / NA	30	287 (TAG→TAA)	qorB	NAD(P)H:quinone oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/14); total (16/14)

AACAGGGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCC  >  NC_000913/4433028‑4433293

aaCAGGGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAattaatta                                                                                                                                 <  1:174633/139‑1 (MQ=255)

   aGGGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGtt                                                                                                                              >  2:137348/1‑139 (MQ=255)

    gggCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGtta                                                                                                                             >  1:372851/1‑139 (MQ=255)

     ggCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGttat                                                                                                                            <  1:450426/139‑1 (MQ=255)

                   cgcTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGa                                                                                                              >  1:62279/1‑139 (MQ=255)

                               cTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCtt                                                                                                  >  2:272018/1‑139 (MQ=255)

                                    aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTACTTAGTTATTAACATTaaaaa                                                                                                                <  2:454313/120‑1 (MQ=255)

                                    aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTaa                                                                                                                         >  1:266064/1‑111 (MQ=255)

                                    aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTaa                                                                                                                         <  2:266064/111‑1 (MQ=255)

                                    aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTaagaa                                                                                                                >  1:454313/1‑120 (MQ=255)

                                    aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGc                                                                                             >  2:78108/1‑139 (MQ=255)

                                    aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGc                                                                                             >  1:518321/1‑139 (MQ=255)

                                       cATTGATCTGTAAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTATTTAGTTATTAACATTAAAAAGATGGCTTACGCGTTCGGCTaa                                                                                          >  2:451130/1‑139 (MQ=255)

                                         ttGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACg                                                                                        >  2:41627/1‑139 (MQ=255)

                                                                     cAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCt                                                            <  1:477509/139‑1 (MQ=255)

                                                                     cAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCt                                                            <  2:104739/139‑1 (MQ=255)

                                                                       gACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTaa                                                          <  1:137348/139‑1 (MQ=255)

                                                                               tGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTaaaa                                                                                                                 <  1:404031/76‑1 (MQ=255)

                                                                               tGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTaaaa                                                                                                                 >  2:404031/1‑76 (MQ=255)

                                                                                gCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGc                                                 <  2:518321/139‑1 (MQ=255)

                                                                                 cTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                                <  1:436293/139‑1 (MQ=255)

                                                                                  tCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCca                                                                       <  1:620302/115‑1 (MQ=255)

                                                                                  tCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCca                                                                       >  2:620302/1‑115 (MQ=255)

                                                                                           cccGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGttatta                                                                                                                          >  2:352090/1‑55 (MQ=255)

                                                                                           cccGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGttatta                                                                                                                          <  1:352090/55‑1 (MQ=255)

                                                                                                          gagGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGAt                       <  1:644397/139‑1 (MQ=255)

                                                                                                             gATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGt                                                                            >  1:767434/1‑83 (MQ=255)

                                                                                                             gATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGt                                                                            <  2:767434/83‑1 (MQ=255)

                                                                                                             gATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATgcg                    >  1:513002/1‑139 (MQ=255)

                                                                                                                  gggAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAAc               >  2:480673/1‑139 (MQ=255)

                                                                                                                               tttAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGcc  <  1:41627/139‑1 (MQ=255)

AACAGGGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCC  >  NC_000913/4433028‑4433293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A