Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,605,605	G→A	P20L (CCG→CTG)	fhuF ←	ferric iron reductase involved in ferric hydroximate transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,605,605	0	G	A	98.1%	166.2 / ‑5.3	52	P20L (CCG→CTG)	fhuF	ferric iron reductase involved in ferric hydroximate transport
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/29); minor base C (0/1); total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTAT  >  NC_000913/4605469‑4605736

cacaTTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGTGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGc                                                                                                                                   >  1:663900/1‑139 (MQ=255)

     tCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGgag                                                                                                                              >  2:286040/1‑139 (MQ=255)

     tCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGgag                                                                                                                              <  2:769207/139‑1 (MQ=255)

         tgatgaCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGc                                                                                                                          >  1:699830/1‑139 (MQ=255)

            tgaCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGtt                                                                                                                       <  2:7919/139‑1 (MQ=255)

             gaCCATTTCGCCAGCGTCATGGCGTTAAGTGGTGCAGGTTCATCCAGTCGGATAAACTCGAGCAAATGTTCACGATGATTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTc                                                                                                                      >  2:488400/1‑139 (MQ=255)

             gaCCATTGCGCCAGCGTCATGGCGTTAAGGGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTc                                                                                                                      >  2:595995/1‑139 (MQ=255)

              aCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCg                                                                                                                     >  1:723282/1‑139 (MQ=255)

                   tGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGa                                                                                                                >  2:614762/1‑139 (MQ=255)

                     cgcCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATg                                                                                                              >  2:512601/1‑139 (MQ=255)

                      gcCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGa                                                                                                             <  1:614762/139‑1 (MQ=255)

                      gcCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGa                                                                                                             <  2:558485/139‑1 (MQ=255)

                         aGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACAt                                                                                                          <  1:404525/139‑1 (MQ=255)

                                   cGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATagag                                                                                                <  2:721029/139‑1 (MQ=255)

                                   cGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATagag                                                                                                <  2:699830/139‑1 (MQ=255)

                                        aGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTg                                                                                           >  2:229250/1‑139 (MQ=255)

                                                aGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGa                                                                                   <  2:184958/139‑1 (MQ=255)

                                                 ggTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGAt                                                                                  <  1:455925/139‑1 (MQ=255)

                                                  gTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCCGCTGGATATGCGTTCGCCAGATTACATCTTCATAGAGCGGTGCGGAACGATa                                                                                 <  2:445955/139‑1 (MQ=255)

                                                  gTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATa                                                                                 >  2:7267/1‑139 (MQ=255)

                                                    tCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAgg                                                                               >  2:134575/1‑139 (MQ=255)

                                                       tCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCa                                                                            >  2:553377/1‑139 (MQ=255)

                                                           ggCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAAt                                                                        <  1:387420/139‑1 (MQ=255)

                                                            gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACAt                                                                                                          <  1:367019/104‑1 (MQ=255)

                                                            gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACAt                                                                                                          >  2:367019/1‑104 (MQ=255)

                                                            gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATa                                                                                                   >  1:545514/1‑111 (MQ=255)

                                                            gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATc                                                                       >  1:492111/1‑139 (MQ=255)

                                                            gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTCGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATa                                                                                                   <  2:545514/111‑1 (MQ=255)

                                                                 tAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGAt                                                                  <  1:553377/139‑1 (MQ=255)

                                                                  aaaCTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATa                                                                 <  1:7267/139‑1 (MQ=255)

                                                                     cTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTa                                                              <  1:90019/139‑1 (MQ=255)

                                                                       ccAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAAt                                                            <  1:286040/139‑1 (MQ=255)

                                                                       ccAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAAt                                                            <  1:282725/139‑1 (MQ=255)

                                                                         aGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCt                                                          <  2:723282/139‑1 (MQ=255)

                                                                                   cACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataat                                                <  1:134575/139‑1 (MQ=255)

                                                                                     cGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataatga                                              >  2:396282/1‑139 (MQ=255)

                                                                                     cGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataatga                                              <  2:240599/139‑1 (MQ=255)

                                                                                                    gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGTGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCg                               >  1:579548/1‑139 (MQ=255)

                                                                                                    gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGa                                                                                   >  2:336504/1‑87 (MQ=255)

                                                                                                    gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGa                                                                                   <  1:336504/87‑1 (MQ=255)

                                                                                                    gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCg                               >  2:281165/1‑139 (MQ=255)

                                                                                                    gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCg                               <  1:512601/139‑1 (MQ=255)

                                                                                                     tcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGa                              <  2:398904/139‑1 (MQ=255)

                                                                                                            cACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGttt                       >  1:357271/1‑139 (MQ=255)

                                                                                                                gCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACa                                                                                                           >  2:208258/1‑51 (MQ=255)

                                                                                                                gCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACa                                                                                                           <  1:208258/51‑1 (MQ=255)

                                                                                                                   tGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGTGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGAt                <  2:579548/139‑1 (MQ=255)

                                                                                                                        aaGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCaa           <  1:77838/139‑1 (MQ=255)

                                                                                                                           cGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGAt                                                                  <  2:592336/81‑1 (MQ=255)

                                                                                                                           cGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGAt                                                                  >  1:592336/1‑81 (MQ=255)

                                                                                                                            gTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGa       <  2:492111/139‑1 (MQ=255)

                                                                                                                                 aTCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTAt  <  1:590804/139‑1 (MQ=255)

CACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTAT  >  NC_000913/4605469‑4605736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A