Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	384,059	C→T	186 (TAG→TAA)	yaiS ←	putative PIG‑L family deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	384,059	0	C	T	100.0%	96.6 / NA	31	186 (TAG→TAA)	yaiS	putative PIG‑L family deacetylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/11); total (20/11)

CCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAG  >  NC_000913/383924‑384194

ccAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAg                                                                                                                                      >  2:641064/1‑139 (MQ=255)

  aGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCt                                                                                                                                    >  2:192430/1‑139 (MQ=255)

         cATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGt                                                                                                                             >  2:271801/1‑139 (MQ=255)

                 ttGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTaa                                                                                                                     <  2:90524/139‑1 (MQ=255)

                   gCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATg                                                                                                                   <  1:192430/139‑1 (MQ=255)

                     tCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgca                                                                                                                 <  1:271801/139‑1 (MQ=255)

                                 tttATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACt                                                                                                     <  2:416892/139‑1 (MQ=255)

                                       aaTTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgcagc                                                                                                               >  1:615897/1‑123 (MQ=255)

                                       aaTTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATgcagc                                                                                                               <  2:615897/123‑1 (MQ=255)

                                           aCAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAtt                                                                                           <  2:678897/139‑1 (MQ=255)

                                                 ccTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATg                                                                                                                              <  1:294745/98‑1 (MQ=255)

                                                 ccTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATg                                                                                                                              >  2:294745/1‑98 (MQ=255)

                                                    tCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                          >  1:443673/1‑131 (MQ=255)

                                                    tCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                          <  2:443673/131‑1 (MQ=255)

                                                     cACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAAc                                                                                 >  2:582141/1‑139 (MQ=255)

                                                           gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTc                                                                           >  1:632355/1‑139 (MQ=255)

                                                           gATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTc                                                                           >  2:10073/1‑139 (MQ=255)

                                                                      aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                          >  1:398967/1‑113 (MQ=255)

                                                                      aCCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATAttc                                                                                          <  2:398967/113‑1 (MQ=255)

                                                                           ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                               <  1:285404/119‑1 (MQ=255)

                                                                           ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACgg                                                                               >  2:285404/1‑119 (MQ=255)

                                                                           ttgtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATa                                                           >  2:627134/1‑139 (MQ=255)

                                                                             gtCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATaaa                                                         >  1:737873/1‑139 (MQ=255)

                                                                                          tgCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTaa                                                                                  >  2:160055/1‑101 (MQ=255)

                                                                                          tgCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTaa                                                                                  <  1:160055/101‑1 (MQ=255)

                                                                                                  cACGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGt                                    >  1:709295/1‑139 (MQ=255)

                                                                                                    cGCAGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGttt                                  >  2:75503/1‑139 (MQ=255)

                                                                                                       aGGCGATCATGACGCATATAATCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGt                               >  1:29201/1‑139 (MQ=255)

                                                                                                                           aTCGCGTCGTTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATa           >  1:633794/1‑139 (MQ=255)

                                                                                                                               cgtcgtTCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTc       >  1:444468/1‑139 (MQ=255)

                                                                                                                                    ttCTTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCggcag  >  2:266426/1‑139 (MQ=255)

CCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCATATAATCGCGTCGTTCCTAGCTTTTATGTTTTTTTAATGCAGCAAGTTTGACTGTGAAATATTCTTCTTTAACGGATTCAAAAACCTGAGGCATAAATGAAAGCCAGGTACTTGGGGTTTCGTAGCCGAGAATTTGCGGAATAGTTCGGCAG  >  NC_000913/383924‑384194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A