Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,029,450	C→T	V174I (GTC→ATC)	rlmI ←	23S rRNA m(5)C1962 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,029,450	0	C	T	100.0%	129.5 / NA	41	V174I (GTC→ATC)	rlmI	23S rRNA m(5)C1962 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/23); total (18/23)

GCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCA  >  NC_000913/1029319‑1029580

gcggcgGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGccc                                                                                                                             >  2:107650/1‑139 (MQ=255)

                 ggcTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCCTCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACGCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTcc                                                                                                            <  2:603882/139‑1 (MQ=255)

                     aTCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCcttct                                                                                                        <  1:65970/139‑1 (MQ=255)

                             ggTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACg                                                                                                <  2:94785/139‑1 (MQ=255)

                              gTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGt                                                                                               >  1:745589/1‑139 (MQ=255)

                                    gtagtaGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACcgcg                                                                                         <  2:614855/139‑1 (MQ=255)

                                        tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTa                                                                                                  <  2:112428/126‑1 (MQ=255)

                                        tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTa                                                                                                  >  1:112428/1‑126 (MQ=255)

                                        tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGt                                                                                     >  1:373104/1‑139 (MQ=255)

                                        tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGt                                                                                     >  2:692189/1‑139 (MQ=255)

                                        tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGt                                                                                     >  2:450027/1‑139 (MQ=255)

                                         agCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTc                                                                                    >  2:27465/1‑139 (MQ=255)

                                           cccGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGc                                                                                  >  2:202891/1‑139 (MQ=255)

                                             cGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTg                                                                                >  2:414819/1‑139 (MQ=255)

                                             cGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTg                                                                                >  2:476628/1‑139 (MQ=255)

                                                  tgtgTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTcc                                                                                                            <  1:74132/106‑1 (MQ=255)

                                                  tgtgTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATc                                                                           <  1:450027/139‑1 (MQ=255)

                                                  tgtgTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATc                                                                           <  1:476628/139‑1 (MQ=255)

                                                  tgtgTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATc                                                                           <  1:242153/139‑1 (MQ=255)

                                                  tgtgTCCGTGCTGAATATCCACCAGCAGCTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGCCCCTGGGTCAGCTACATTcc                                                                                                            >  2:74132/1‑106 (MQ=255)

                                                      tCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTaa                                                                       >  2:457965/1‑139 (MQ=255)

                                                          tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACcgc                                                                                          >  1:143544/1‑116 (MQ=255)

                                                          tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACcgc                                                                                          <  2:143544/116‑1 (MQ=255)

                                                          tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCg                                                                   >  2:733950/1‑139 (MQ=255)

                                                          tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCg                                                                   <  1:692189/139‑1 (MQ=255)

                                                          tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCg                                                                   >  1:431211/1‑139 (MQ=255)

                                                           gCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGa                                                                  <  1:540115/139‑1 (MQ=255)

                                                                      accaGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGTTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       <  1:518704/139‑1 (MQ=255)

                                                                      accaGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       <  1:457965/139‑1 (MQ=255)

                                                                      accaGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       <  1:107650/139‑1 (MQ=255)

                                                                         agcagTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       >  1:478244/1‑136 (MQ=255)

                                                                         agcagTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       <  2:478244/136‑1 (MQ=255)

                                                                                  aTTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCa                                           <  2:13861/139‑1 (MQ=255)

                                                                                  aTTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCa                                           <  2:373104/139‑1 (MQ=255)

                                                                                     ccGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAggg                                        <  2:431211/139‑1 (MQ=255)

                                                                                                      cagcagGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCcgcg                       <  1:698123/139‑1 (MQ=255)

                                                                                                                       aCTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCa                                           <  2:40269/102‑1 (MQ=255)

                                                                                                                       aCTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCa                                           >  1:40269/1‑102 (MQ=255)

                                                                                                                           gCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTc            >  2:440382/1‑129 (MQ=255)

                                                                                                                           gCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTc            <  1:440382/129‑1 (MQ=255)

                                                                                                                           gCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCa  <  1:740619/139‑1 (MQ=255)

GCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCA  >  NC_000913/1029319‑1029580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A