Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
757 |
38.1 |
1607544 |
96.5% |
1551279 |
117.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,164,998 |
G→A |
*241* (TAG→TAA) |
baeR → |
response regulator in two‑component regulatory system with BaeS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,164,998 | 0 | G | A | 100.0%
| 76.8
/ NA
| 25 | *241* (TAG→TAA) | baeR | response regulator in two‑component regulatory system with BaeS |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/14); total (11/14) |
CCGACCGCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATAC > NC_000913/2164865‑2165124
|
ccgaccgCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTa < 2:437143/139‑1 (MQ=255)
gCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGCGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACa > 1:358236/1‑139 (MQ=255)
cGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTAtttt < 1:625005/139‑1 (MQ=255)
ccACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGc < 2:794055/139‑1 (MQ=255)
cTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGtt < 2:410463/139‑1 (MQ=255)
tGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTc > 1:313452/1‑139 (MQ=255)
tGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTc > 2:682494/1‑139 (MQ=255)
cgccgcAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGa > 1:58190/1‑108 (MQ=255)
cgccgcAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGa < 2:58190/108‑1 (MQ=255)
aaGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCTTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGa < 2:313452/139‑1 (MQ=255)
tGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCACATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCg < 2:528340/139‑1 (MQ=255)
aaTCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTTAGACCAGATCCGGCa < 1:742082/139‑1 (MQ=255)
ctcGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACa < 1:75637/92‑1 (MQ=255)
ctcGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACa > 2:75637/1‑92 (MQ=255)
gAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATc < 2:515244/133‑1 (MQ=255)
gAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATc > 1:515244/1‑133 (MQ=255)
gcgcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGa > 1:780376/1‑139 (MQ=255)
gcgcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGa < 2:496563/139‑1 (MQ=255)
cGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACAtt > 2:744438/1‑139 (MQ=255)
gTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAg > 2:192144/1‑117 (MQ=255)
gTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAg < 1:192144/117‑1 (MQ=255)
cGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGccc > 2:694991/1‑139 (MQ=255)
ttACCGCTGCGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTgg < 2:358236/139‑1 (MQ=255)
gCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGaaa < 1:682494/139‑1 (MQ=255)
ccGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATAc > 2:98498/1‑139 (MQ=255)
|
CCGACCGCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATAC > NC_000913/2164865‑2165124
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
