Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,398,485	C→T	A561T (GCC→ACC)	nuoG ←	NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,398,485	0	C	T	100.0%	114.6 / NA	36	A561T (GCC→ACC)	nuoG	NADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/15); total (21/15)

GGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGA  >  NC_000913/2398353‑2398619

gggCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTcc                                                                                                                                  <  1:253519/139‑1 (MQ=255)

                  cTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGt                                                                                                                               <  2:480251/124‑1 (MQ=255)

                  cTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGt                                                                                                                               >  1:480251/1‑124 (MQ=255)

                  cTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTaa                                                                                                                >  1:699538/1‑139 (MQ=255)

                  cTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTaa                                                                                                                >  1:515734/1‑139 (MQ=255)

                  cTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTaa                                                                                                                >  2:803000/1‑139 (MQ=255)

                   tgtgCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCGCGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGt                                                                                                                                    <  2:583335/118‑1 (MQ=255)

                   tgtgCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCGCGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGt                                                                                                                                    >  1:583335/1‑118 (MQ=255)

                     tgCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAAc                                                                                                               >  1:200351/1‑137 (MQ=255)

                     tgCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAAc                                                                                                               <  2:200351/137‑1 (MQ=255)

                                    ccaccaTCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAAcc                                                                                              >  1:196353/1‑139 (MQ=255)

                                     caccaTCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCg                                                                                             <  1:408851/139‑1 (MQ=255)

                                      accaTCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGc                                                                                            >  1:368145/1‑139 (MQ=255)

                                           cACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTc                                                                                                                                   <  2:702285/95‑1 (MQ=255)

                                           cACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTc                                                                                                                                   >  1:702285/1‑95 (MQ=255)

                                                   gTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGcc                                                                               <  2:368145/139‑1 (MQ=255)

                                                      cTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTa                                                                                                                 <  1:457557/102‑1 (MQ=255)

                                                      cTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTa                                                                                                                 >  2:457557/1‑102 (MQ=255)

                                                                cgcAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAACCGAACCGCCACCCATAATGCCCAGCCCCATGCTg                                                                  >  1:34995/1‑139 (MQ=255)

                                                                             cgGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACggg                                                     <  2:683124/139‑1 (MQ=255)

                                                                             cgGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACggg                                                     <  2:515734/139‑1 (MQ=255)

                                                                                   gCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCa                                               <  1:803000/139‑1 (MQ=255)

                                                                                            tGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATa                                                                                    <  1:288464/93‑1 (MQ=255)

                                                                                            tGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATa                                                                                    >  2:288464/1‑93 (MQ=255)

                                                                                              aCGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCg                                    >  2:260042/1‑139 (MQ=255)

                                                                                                  tGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAAt                                                 >  2:88677/1‑122 (MQ=255)

                                                                                                  tGCAGATCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAAt                                                 <  1:88677/122‑1 (MQ=255)

                                                                                                       aTCGTTTTCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAgcg                           >  2:794748/1‑139 (MQ=255)

                                                                                                              tCCAACACCACCACCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGAc                    >  2:802012/1‑139 (MQ=255)

                                                                                                                   caccaccacCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGcc                                                                               >  1:758055/1‑75 (MQ=255)

                                                                                                                   caccaccacCGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGcc                                                                               <  2:758055/75‑1 (MQ=255)

                                                                                                                     ccaccaccGCGTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAg             >  1:231324/1‑139 (MQ=255)

                                                                                                                              cgTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATgg                                            <  2:309920/99‑1 (MQ=255)

                                                                                                                              cgTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATgg                                            >  1:309920/1‑99 (MQ=255)

                                                                                                                              cgTCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTcgc    >  2:51774/1‑139 (MQ=255)

                                                                                                                                tCGGTGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGa  >  2:106577/1‑139 (MQ=255)

GGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGA  >  NC_000913/2398353‑2398619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A