Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,981,901	(C)_5→6	coding (282/1419 nt)	araE ←	arabinose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,981,896	1	.	C	100.0%	180.4 / NA	50	A96G (GCC→GGC)	araE	arabinose transporter
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (26/24); total (26/24)

TGCCATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑CCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCAC  >  NC_000913/2981760‑2982020

tGCCATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑cc                                                                                                                            >  2:256377/1‑139 (MQ=255)

     tttCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑ccccccg                                                                                                                       <  2:150425/139‑3 (MQ=255)

        cAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑ccccccgcca                                                                                                                    <  1:203353/139‑6 (MQ=255)

         aGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCAt                                                                                                                    <  1:227600/139‑1 (MQ=255)

         aGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCAt                                                                                                                    <  1:553580/139‑1 (MQ=255)

             aGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAgg                                                                                                                <  1:256377/139‑1 (MQ=255)

               aTACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCt                                                                                                              <  1:56849/139‑1 (MQ=255)

                        agCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACg                                                                                                     >  1:539131/1‑139 (MQ=255)

                         gCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGc                                                                                                    >  1:790873/1‑139 (MQ=255)

                          cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGcc                                                                                                   <  2:539131/139‑1 (MQ=255)

                          cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGcc                                                                                                   >  1:385482/1‑139 (MQ=255)

                                       gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCAt                                                                                                                    <  2:520011/109‑1 (MQ=255)

                                       gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCAt                                                                                                                    >  1:520011/1‑109 (MQ=255)

                                       gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGa                                                                                      >  2:421700/1‑139 (MQ=255)

                                       gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGa                                                                                      >  1:257508/1‑139 (MQ=255)

                                         tCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACa                                                                                    >  2:153405/1‑139 (MQ=255)

                                            cGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 <  2:5574/139‑1 (MQ=255)

                                                   aaTGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCAtt                                                                          <  1:421700/139‑1 (MQ=255)

                                                     tGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaa                                                                        >  1:30611/1‑139 (MQ=255)

                                                               cacaCGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCAcc                                                              <  2:257508/139‑1 (MQ=255)

                                                               cacaCGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCAcc                                                              <  1:514853/139‑1 (MQ=255)

                                                                 cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCaa                                                            >  1:158531/1‑139 (MQ=255)

                                                                 cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCaa                                                            >  2:554556/1‑139 (MQ=255)

                                                                      gcggcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                       <  1:504442/139‑1 (MQ=255)

                                                                      gcggcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                       >  1:610886/1‑139 (MQ=255)

                                                                         gcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCa                                                    >  1:381936/1‑139 (MQ=255)

                                                                         gcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCa                                                    <  2:30611/139‑1 (MQ=255)

                                                                                 cATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcat                                            <  2:790873/139‑1 (MQ=255)

                                                                                   tctcTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcatca                                          <  1:257103/139‑1 (MQ=255)

                                                                                     tctACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATg                                        <  1:197993/139‑1 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 <  2:330630/89‑1 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 >  1:330630/1‑89 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 <  1:496289/89‑1 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 >  2:496289/1‑89 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCaa                                                                               >  1:733475/1‑91 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCaa                                                                               <  2:733475/91‑1 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGGGCATCATGCTACTAacc                               >  2:666505/1‑139 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                               >  1:644173/1‑123 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                               <  2:644173/123‑1 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacc                               >  1:161022/1‑139 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacc                               >  1:529865/1‑139 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑ccccccg                                                                                                                       >  1:565917/1‑48 (MQ=255)

                                                                                              gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑ccccccg                                                                                                                       <  2:565917/50‑3 (MQ=255)

                                                                                               tCGCAAAAGCGGACCATATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacca                              >  1:537668/1‑139 (MQ=255)

                                                                                                 gCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccacc                            <  1:554556/139‑1 (MQ=255)

                                                                                                   aaaaGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCa                          >  2:193038/1‑139 (MQ=255)

                                                                                                                    aaCCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTgg         <  1:56505/139‑1 (MQ=255)

                                                                                                                     aCCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGt        <  2:610886/139‑1 (MQ=255)

                                                                                                                      ccGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTc       <  1:193038/139‑1 (MQ=255)

                                                                                                                         aGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGc    >  2:200307/1‑139 (MQ=255)

                                                                                                                         aGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGc    >  2:584558/1‑139 (MQ=255)

                                                                                                                         aGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGc    >  2:634380/1‑139 (MQ=255)

                                                                                                                           tACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGcac  >  2:479708/1‑139 (MQ=255)

                                                                                                                                    gATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAgcg                                                                  >  1:88198/1‑66 (MQ=255)

                                                                                                                                    gATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAgcg                                                                  <  2:88198/66‑1 (MQ=255)

TGCCATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑CCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCAC  >  NC_000913/2981760‑2982020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A