Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I2 R1 757 38.1 1607544 96.5% 1551279 117.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,299,438 A→G T155T (ACA→ACG)  yhbO → stress‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,299,4380AG100.0% 63.1 / NA 19T155T (ACA→ACG) yhbOstress‑resistance protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

TCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGG  >  NC_000913/3299305‑3299562
                                                                                                                                     |                                                                                                                            
tCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa                                                                                                                         <  2:26454/139‑1 (MQ=255)
        gATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAAACGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGc                                                                                                                 <  1:153262/139‑1 (MQ=255)
                    gggCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAAcc                                                                                                     >  1:61668/1‑139 (MQ=255)
                                                 cATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGa                                                                        <  1:262334/139‑1 (MQ=255)
                                                 cATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGa                                                                        >  2:359359/1‑139 (MQ=255)
                                                      ttGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTcgc                                                                   <  2:61668/139‑1 (MQ=255)
                                                              aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGcc                                                                                                                <  1:507194/86‑1 (MQ=255)
                                                              aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGcc                                                                                                                >  2:507194/1‑86 (MQ=255)
                                                              aaaaaTGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCaa                                                           >  1:354831/1‑139 (MQ=255)
                                                                     cGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAttt                                                    >  1:327093/1‑139 (MQ=255)
                                                                     cGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAttt                                                    >  2:472550/1‑139 (MQ=255)
                                                                      gggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACcgcg                                                                                                  <  2:748552/92‑1 (MQ=255)
                                                                      gggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACcgcg                                                                                                  >  1:748552/1‑92 (MQ=255)
                                                                      gggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAtttt                                                   >  1:718359/1‑139 (MQ=255)
                                                                               aTTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCaaa                                          <  2:718359/139‑1 (MQ=255)
                                                                                          aGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCgtgt                               >  1:571/1‑139 (MQ=255)
                                                                                                    ggTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGaa                     >  2:672964/1‑139 (MQ=255)
                                                                                                                       gTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCgg  >  2:570942/1‑139 (MQ=255)
                                                                                                                       gTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCgg  <  1:359359/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                            
TCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGG  >  NC_000913/3299305‑3299562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A