Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,299,438 |
A→G |
T155T (ACA→ACG) |
yhbO → |
stress‑resistance protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,299,438 | 0 | A | G | 100.0%
| 143.4
/ NA
| 41 | T155T (ACA→ACG) | yhbO | stress‑resistance protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/18); total (23/18) |
GCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACA > NC_000913/3299308‑3299572
|
gcgcCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGa > 1:518463/1‑139 (MQ=255)
cgcCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGAt > 2:1222758/1‑139 (MQ=255)
gATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGc < 1:1222758/139‑1 (MQ=255)
aTTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGt > 1:418357/1‑139 (MQ=255)
ggggCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa > 2:870000/1‑120 (MQ=255)
ggggCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa < 1:870000/120‑1 (MQ=255)
cGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa < 2:474541/129‑1 (MQ=255)
cGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa > 1:474541/1‑129 (MQ=255)
aTCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCt > 2:332928/1‑139 (MQ=255)
cATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGa > 1:133503/1‑139 (MQ=255)
gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa > 1:1328466/1‑90 (MQ=255)
gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa < 2:1328466/90‑1 (MQ=255)
gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGc > 1:687390/1‑139 (MQ=255)
cGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑ttt > 1:979962/1‑139 (MQ=255)
gAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCa < 2:518463/139‑1 (MQ=255)
ttACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGcc < 2:687390/139‑1 (MQ=255)
aGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCTTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCgtgt > 2:1098191/1‑139 (MQ=255)
gTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCg < 1:255017/139‑1 (MQ=255)
tCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa < 2:157311/67‑1 (MQ=255)
tCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa > 1:157311/1‑67 (MQ=255)
tGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa < 1:149466/50‑1 (MQ=255)
tGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa > 2:149466/1‑50 (MQ=255)
tGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa > 1:284995/1‑64 (MQ=255)
tGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa < 2:284995/64‑1 (MQ=255)
gATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt < 1:199119/64‑1 (MQ=255)
gATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt > 2:199119/1‑64 (MQ=255)
tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa < 1:807897/43‑1 (MQ=255)
tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa > 2:807897/1‑43 (MQ=255)
aGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTc > 2:1429718/1‑139 (MQ=255)
cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAg < 2:286434/139‑1 (MQ=255)
cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGtgttgt < 2:1406232/116‑1 (MQ=255)
cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGtgttgt > 1:1406232/1‑116 (MQ=255)
cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAAtt < 2:584300/125‑1 (MQ=255)
cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAAtt > 1:584300/1‑125 (MQ=255)
cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGc > 2:656514/1‑139 (MQ=255)
tGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGcc < 1:656514/139‑1 (MQ=255)
tGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTAGATTTCATCGAGcc < 1:933618/139‑1 (MQ=255)
tACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCaaa < 2:206661/97‑1 (MQ=255)
tACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCaaa > 1:206661/1‑97 (MQ=255)
cAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTc > 2:1337409/1‑139 (MQ=255)
ccggacgccggacgATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCca > 2:1408191/1‑139 (MQ=255)
ggACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCcaca > 2:755534/1‑139 (MQ=255)
|
GCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACA > NC_000913/3299308‑3299572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
