Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,801	G→A	T66I (ACA→ATA)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,801	0	G	A	100.0%	107.3 / NA	34	T66I (ACA→ATA)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/11); total (23/11)

CGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTT  >  NC_000913/3379666‑3379921

cGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTatgt                                                                                                                       >  2:351698/1‑139 (MQ=255)

  cagcaTAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCt                                                                                                                     >  2:177394/1‑139 (MQ=255)

         aaaCGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGtt                                                                                                              <  2:378689/139‑1 (MQ=255)

             ggTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTAcc                                                                                                          <  2:371118/139‑1 (MQ=255)

               tGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTAcccc                                                                                                        >  2:454137/1‑139 (MQ=255)

                               gTTTCCGCTCTCCCGGCAGTCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACCCCGCCCCACATTTATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCcg                                                                                        <  1:748336/139‑1 (MQ=255)

                                        ctcCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAg                                                                               >  2:714198/1‑139 (MQ=255)

                                                   cTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTcc                                                                    >  1:303651/1‑139 (MQ=255)

                                                     ttGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTg                                                                  >  1:40573/1‑139 (MQ=255)

                                                              aaGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCtggt                                                         >  2:399109/1‑139 (MQ=255)

                                                                  tCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCg                                                     <  2:319589/139‑1 (MQ=255)

                                                                    cATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTg                                                   <  1:678686/139‑1 (MQ=255)

                                                                            aGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATtgat                                           <  1:635581/139‑1 (MQ=255)

                                                                            aGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATtgat                                           >  1:88211/1‑139 (MQ=255)

                                                                                                   ccccACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCa                    >  2:793027/1‑139 (MQ=255)

                                                                                                    cccACATATATAAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAg                   >  2:553991/1‑139 (MQ=255)

                                                                                                            tataAGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGa           <  2:303651/139‑1 (MQ=255)

                                                                                                               aaGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTa                                                                                <  2:390400/67‑1 (MQ=255)

                                                                                                               aaGCCACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTa                                                                                >  1:390400/1‑67 (MQ=255)

                                                                                                                  ccACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCct     >  1:727089/1‑139 (MQ=255)

                                                                                                                  ccACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCct     >  1:242303/1‑139 (MQ=255)

                                                                                                                   cACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCctc    >  2:505640/1‑139 (MQ=255)

                                                                                                                   cACGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCctc    >  1:786598/1‑139 (MQ=255)

                                                                                                                    aCGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCctct   >  2:96041/1‑139 (MQ=255)

                                                                                                                    aCGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCctct   >  2:16062/1‑139 (MQ=255)

                                                                                                                     cGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCcgc                                                                                       >  2:12443/1‑54 (MQ=255)

                                                                                                                     cGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCcgc                                                                                       <  1:12443/54‑1 (MQ=255)

                                                                                                                     cGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCAc                                                                          <  2:453455/67‑1 (MQ=255)

                                                                                                                     cGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCAc                                                                          >  1:453455/1‑67 (MQ=255)

                                                                                                                     cGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCtt  >  2:322135/1‑139 (MQ=255)

                                                                                                                     cGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCtt  >  2:151732/1‑139 (MQ=255)

                                                                                                                     cGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCtt  >  2:115911/1‑139 (MQ=255)

                                                                                                                     cGCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCtt  >  1:793338/1‑139 (MQ=255)

                                                                                                                      gCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCca                                                                                                       >  2:276869/1‑37 (MQ=255)

                                                                                                                      gCACTGGCGTATGCTTTATGTCTTCGCGTTTACCCca                                                                                                       <  1:276869/37‑1 (MQ=255)

CGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTTTCCCGCGTCCTACACCGCCCCACATATATAAGCCACGCACTGGCGTATGCTTTGTGTCTTCGCGTTTACCCCACAGCTTACCGACCCGCGCCATTAGCCCACTCGTCCTGGGGGCTGGTGGCGTGCTATTGATGAGTTCCTGATAAATAATTTCCAGGCGGCTGACGGCCTCTT  >  NC_000913/3379666‑3379921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A