Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,857	C→T	91 (TAG→TAA)	yhcO ←	putative barnase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,857	0	C	T	100.0%	100.9 / NA	32	91 (TAG→TAA)	yhcO	putative barnase inhibitor
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/13); total (19/13)

CGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCAGG  >  NC_000913/3385729‑3385992

cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGaa                                                                                                                               >  2:439201/1‑139 (MQ=255)

                         gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAg                                                                                                      <  2:7361/139‑1 (MQ=255)

                              tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTATTGTGCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAGACGCAAATGACCTTCCAGCTCtt                                                                                                 >  1:528049/1‑139 (MQ=255)

                                         ccTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtca                                                                                      <  2:397942/139‑1 (MQ=255)

                                                  cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGc                                                                             >  1:455729/1‑139 (MQ=255)

                                                  cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGc                                                                             >  2:112618/1‑139 (MQ=255)

                                                       gCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACg                                                                                                                                 >  2:651250/1‑82 (MQ=255)

                                                       gCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACg                                                                                                                                 <  1:651250/82‑1 (MQ=255)

                                                               gTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                                                 >  2:750838/1‑122 (MQ=255)

                                                               gTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                                                 <  1:750838/122‑1 (MQ=255)

                                                                tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                           >  1:549749/1‑111 (MQ=255)

                                                                tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                           <  2:549749/111‑1 (MQ=255)

                                                                tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGcc                                                               >  2:759076/1‑139 (MQ=255)

                                                                     aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAAc                                                          >  1:385430/1‑139 (MQ=255)

                                                                          cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                                                     >  1:248052/1‑139 (MQ=255)

                                                                          cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                                                     >  2:692186/1‑139 (MQ=255)

                                                                          cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                                                     >  2:582719/1‑139 (MQ=255)

                                                                             tCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATg                                                                                                               >  1:146830/1‑78 (MQ=255)

                                                                             tCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATg                                                                                                               <  2:146830/78‑1 (MQ=255)

                                                                              cATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgcg                                                 <  1:692186/139‑1 (MQ=255)

                                                                               aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAAt                                                                                                                >  1:665567/1‑75 (MQ=255)

                                                                               aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAAt                                                                                                                <  2:665567/75‑1 (MQ=255)

                                                                                  gTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                             <  2:680568/139‑1 (MQ=255)

                                                                                    cTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                    >  1:588855/1‑82 (MQ=255)

                                                                                    cTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                    <  2:588855/82‑1 (MQ=255)

                                                                                         ccGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTccc                                      >  2:301814/1‑139 (MQ=255)

                                                                                          cgacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCa                                     >  1:584222/1‑139 (MQ=255)

                                                                                                       cccGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                              <  2:569141/85‑1 (MQ=255)

                                                                                                       cccGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                              >  1:569141/1‑85 (MQ=255)

                                                                                                          ggTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAt                     >  1:395138/1‑139 (MQ=255)

                                                                                                                    cTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt           <  1:582719/139‑1 (MQ=255)

                                                                                                                         ttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCgg      <  1:716510/139‑1 (MQ=255)

                                                                                                                             gcgtTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCAgg  >  2:521334/1‑139 (MQ=255)

CGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCAGG  >  NC_000913/3385729‑3385992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A