Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,857	C→T	91 (TAG→TAA)	yhcO ←	putative barnase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,857	0	C	T	100.0%	88.5 / NA	29	91 (TAG→TAA)	yhcO	putative barnase inhibitor
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/14); total (15/14)

AAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGG  >  NC_000913/3385725‑3385988

aaGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGa                                                                                                                               <  1:625438/139‑1 (MQ=255)

                      tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGcc                                                                                                         <  2:279888/139‑1 (MQ=255)

                           aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACCAATAAGCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTAGGGGGCTTTTTTTTGCGTTAATGACGAACATTATAACGCAAATGCCCTTcc                                                                                                    <  1:455876/139‑1 (MQ=255)

                           aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCtcca                                                                                                    >  1:118962/1‑136 (MQ=255)

                             gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAg                                                                                                  >  1:56318/1‑139 (MQ=255)

                              cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc                                                                                                 >  1:320130/1‑139 (MQ=255)

                                cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctc                                                                                               >  2:486096/1‑139 (MQ=255)

                                  tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCCCtt                                                                                             >  1:560470/1‑139 (MQ=255)

                                        tGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                       <  1:299793/139‑1 (MQ=255)

                                         gACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGc                                                                                      <  2:195359/139‑1 (MQ=255)

                                                ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                               <  2:56318/139‑1 (MQ=255)

                                                ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                               <  2:23689/139‑1 (MQ=255)

                                                  cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGcc                                                                                                         >  1:446593/1‑111 (MQ=255)

                                                  cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                                             >  2:66767/1‑139 (MQ=255)

                                                  cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCACGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGcc                                                                                                         <  2:446593/111‑1 (MQ=255)

                                                     gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                          <  1:486096/139‑1 (MQ=255)

                                                      cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                                             >  2:212757/1‑135 (MQ=255)

                                                      cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                                             <  1:212757/135‑1 (MQ=255)

                                                            cTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATAtt                                                                   >  2:177565/1‑139 (MQ=255)

                                                                    tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGcc                                                           >  1:665976/1‑139 (MQ=255)

                                                                    tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGcc                                                           >  1:104261/1‑139 (MQ=255)

                                                                            aaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGc                                                   >  2:105517/1‑139 (MQ=255)

                                                                                           gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctctc                                    <  2:665976/139‑1 (MQ=255)

                                                                                             ccGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                    <  1:502061/73‑1 (MQ=255)

                                                                                             ccGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                    >  2:502061/1‑73 (MQ=255)

                                                                                                acgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATAAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGa                               >  1:441467/1‑139 (MQ=255)

                                                                                                              ggTTCGGGGGCTTTCTTTTGCGTTAATGACGAACATTAAAACGCAACTGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAt                 >  2:321142/1‑139 (MQ=255)

                                                                                                                      ggCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTg         <  2:107985/139‑1 (MQ=255)

                                                                                                                            tttttGCGTTAATGACGAACATTAAAACGCAAATGCCC‑TCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCgg  <  2:118962/139‑1 (MQ=255)

AAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGG  >  NC_000913/3385725‑3385988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A