Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I2 R1 766 64.2 3232166 90.9% 2938038 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,385,857 C→T *91* (TAG→TAA)  yhcO ← putative barnase inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,385,8570CT100.0% 181.4 / NA 57*91* (TAG→TAA) yhcOputative barnase inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/29);  total (28/29)

AAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAA  >  NC_000913/3385724‑3385972
                                                                                                                                      |                                                                                                                   
aaaGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAAtt                                                                                                                >  1:420917/1‑138 (MQ=255)
aaaGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATg                                                                                                                >  1:943736/1‑139 (MQ=255)
     cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACg                                                                                                             <  2:1510307/137‑1 (MQ=255)
     cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACg                                                                                                             >  1:1510307/1‑137 (MQ=255)
                    gATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAAt                                                                                            <  2:1158971/139‑1 (MQ=255)
                       tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGcc                                                                                         >  2:1357899/1‑139 (MQ=255)
                          tGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                      >  1:1365109/1‑139 (MQ=255)
                                tCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                <  1:46648/139‑1 (MQ=255)
                                   tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCtt                                                                             >  1:474936/1‑139 (MQ=255)
                                         tGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                       >  2:735533/1‑139 (MQ=255)
                                            cacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                                    <  1:119368/139‑1 (MQ=255)
                                              ccTGGCACGCTACGGCTGAACTGTAC‑AATAAACCCTCATCGGCCTTTCCGACGAAATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGAAGAACATTTAAACGCAAATGCCCCTCCAGCTTTTCCTCTGCCtcat                                                                 >  2:561192/1‑139 (MQ=255)
                                                tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                      >  2:529620/1‑117 (MQ=255)
                                                tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                      <  1:529620/117‑1 (MQ=255)
                                                tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatc                                                                >  2:830655/1‑139 (MQ=255)
                                                   cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                             <  1:230443/139‑1 (MQ=255)
                                                     cGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACa                                                           <  1:1357899/139‑1 (MQ=255)
                                                      gCTACGGCTGAACTGTACAAATAACCCCTCACCGTTTGGCCCGCGGATATTGCCCCCGGTTCGGGGGT‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  2:420917/139‑1 (MQ=255)
                                                      gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  2:943736/139‑1 (MQ=255)
                                                      gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  1:735533/139‑1 (MQ=255)
                                                      gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  2:1365109/139‑1 (MQ=255)
                                                                     tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATg                                                                                                                <  2:87388/70‑1 (MQ=255)
                                                                     tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATg                                                                                                                >  1:87388/1‑70 (MQ=255)
                                                                          aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCt                                                                                       >  2:1571793/1‑90 (MQ=255)
                                                                          aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCt                                                                                       <  1:1571793/90‑1 (MQ=255)
                                                                             aaCCCTCATCGTCTTGTCCGACGATATTGCGCCCAGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAGCCGCAATATTAACGCGCCAAAACGGc                                   <  1:1188666/139‑1 (MQ=255)
                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAAt                                                                                                                 <  1:1236776/59‑1 (MQ=255)
                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAAt                                                                                                                 >  2:1236776/1‑59 (MQ=255)
                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                    <  1:75206/88‑1 (MQ=255)
                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                    >  2:75206/1‑88 (MQ=255)
                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                >  1:368233/1‑92 (MQ=255)
                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                <  2:368233/92‑1 (MQ=255)
                                                                                  tCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgc                              >  2:661226/1‑139 (MQ=255)
                                                                                    aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                                     >  1:159455/1‑82 (MQ=255)
                                                                                    aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                                     <  2:159455/82‑1 (MQ=255)
                                                                                    aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTaa                                                 <  1:423253/118‑1 (MQ=255)
                                                                                    aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTaa                                                 >  2:423253/1‑118 (MQ=255)
                                                                                    aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGt                            >  2:1252004/1‑139 (MQ=255)
                                                                                            gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                             >  1:1260055/1‑98 (MQ=255)
                                                                                            gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                             <  2:1260055/98‑1 (MQ=255)
                                                                                            gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                            >  2:357085/1‑99 (MQ=255)
                                                                                            gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                            <  1:357085/99‑1 (MQ=255)
                                                                                              ccGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTccc                  <  2:474936/139‑1 (MQ=255)
                                                                                                  cgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          <  1:1172492/95‑1 (MQ=255)
                                                                                                  cgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                          >  2:1172492/1‑95 (MQ=255)
                                                                                                      atTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAAc          >  2:1180794/1‑139 (MQ=255)
                                                                                                       ttGCCCCCGGTTCGGGGGCTTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGttt                          <  2:1397114/123‑1 (MQ=255)
                                                                                                       ttGCCCCCGGTTCGGGGGCTTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGttt                          >  1:1397114/1‑123 (MQ=255)
                                                                                                            cccGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTc    >  2:623461/1‑139 (MQ=255)
                                                                                                              cGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa  <  1:470485/139‑1 (MQ=255)
                                                                                                               ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTcc                                                                           >  2:231761/1‑65 (MQ=255)
                                                                                                               ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTcc                                                                           <  1:231761/65‑1 (MQ=255)
                                                                                                               ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGc                                                         >  2:1295708/1‑83 (MQ=255)
                                                                                                               ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGc                                                         <  1:1295708/83‑1 (MQ=255)
                                                                                                                            tttttttGCGTTAATGACGAACATTAAACCGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                               >  1:826656/1‑65 (MQ=255)
                                                                                                                            tttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                               <  2:826656/65‑1 (MQ=255)
                                                                                                                            tttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                            <  2:731288/68‑1 (MQ=255)
                                                                                                                            tttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                            >  1:731288/1‑68 (MQ=255)
                                                                                                                                      |                                                                                                                   
AAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAA  >  NC_000913/3385724‑3385972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A