Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,727	G→A	P288P (CCG→CCA)	acrE →	cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,727	0	G	A	100.0%	89.5 / NA	29	P288P (CCG→CCA)	acrE	cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/13); total (16/13)

CATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGC  >  NC_000913/3414601‑3414861

cATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTtgtg                                                                                                                            <  1:134227/139‑1 (MQ=255)

                                 tACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGAc                                                                                           >  2:364980/1‑139 (MQ=255)

                                                           ttGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGt                                                                 <  1:414504/139‑1 (MQ=255)

                                                           ttGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGt                                                                 <  2:36190/139‑1 (MQ=255)

                                                                     cACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGcaca                                                       >  2:297297/1‑139 (MQ=255)

                                                                      aCCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTc                                                                                                    >  2:541252/1‑93 (MQ=255)

                                                                      aCCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTc                                                                                                    <  1:541252/93‑1 (MQ=255)

                                                                        cGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACcg                                                    >  1:620659/1‑139 (MQ=255)

                                                                           cTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCgt                                                 <  2:704695/139‑1 (MQ=255)

                                                                                      tACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAAc                                      >  1:307254/1‑139 (MQ=255)

                                                                                        cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGt                                                                                                                                 <  2:81122/46‑1 (MQ=255)

                                                                                        cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGt                                                                                                                                 >  1:81122/1‑46 (MQ=255)

                                                                                        cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGcaac                                                                        >  1:294078/1‑103 (MQ=255)

                                                                                        cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGcaac                                                                        <  2:294078/103‑1 (MQ=255)

                                                                                        cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGt                                                                 <  2:318759/110‑1 (MQ=255)

                                                                                        cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGt                                                                 >  1:318759/1‑110 (MQ=255)

                                                                                        cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCg                                    >  2:186083/1‑139 (MQ=255)

                                                                                              gTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGa                              >  2:354223/1‑139 (MQ=255)

                                                                                                  tCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGcaca                                                       <  1:474774/110‑1 (MQ=255)

                                                                                                  tCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGcaca                                                       >  2:474774/1‑110 (MQ=255)

                                                                                                         cccGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGca                                                         <  2:32301/101‑1 (MQ=255)

                                                                                                         cccGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGca                                                         >  1:32301/1‑101 (MQ=255)

                                                                                                             cAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCt                                                                                              <  1:741721/60‑1 (MQ=255)

                                                                                                             cAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCt                                                                                              >  2:741721/1‑60 (MQ=255)

                                                                                                              aaCATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAg                                                                                                         <  2:237112/48‑1 (MQ=255)

                                                                                                              aaCATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAg                                                                                                         >  1:237112/1‑48 (MQ=255)

                                                                                                                   aCGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGcaac                                                                        <  1:635698/76‑1 (MQ=255)

                                                                                                                   aCGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGcaac                                                                        >  2:635698/1‑76 (MQ=255)

                                                                                                                          tGCCAGGTATGTTTGTGCGTGCACGGAGTGATGAAGGCGTCCAACTTGACGCCCTCCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGGCATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGATTGAAgc  >  1:280897/1‑139 (MQ=255)

CATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGC  >  NC_000913/3414601‑3414861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A