Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
773 |
79.9 |
4112934 |
92.2% |
3792125 |
104.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
509,038 |
G→A |
A781V (GCG→GTG) |
copA ← |
copper transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 509,038 | 0 | G | A | 97.3%
| 115.7
/ NA
| 37 | A781V (GCG→GTG) | copA | copper transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (19/17); minor base C (1/0); total (20/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGGTTGGCGTTACTCACTACGGTAATCGACGAGAGCGCCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACGCACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTGCCGCCACCCATCG > NC_000913/508902‑509173
|
ccGGTTGGCGTTACTCACTACGGTAATCGACGAGAGCGCCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAAcaca < 2:204845/139‑1 (MQ=255)
tACTCACTACGGTAATCGACGAGAGCGCCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGtt < 1:890815/139‑1 (MQ=255)
tACTCACTACGGTAATCGACGAGAGCGCCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGtt < 1:2027591/139‑1 (MQ=255)
tAATCGACGAGAGCGCCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCAtgtt > 2:1853093/1‑139 (MQ=255)
gacgaGAGCGCCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCa > 1:1330660/1‑139 (MQ=255)
gacgaGAGCGCCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCa > 1:345348/1‑139 (MQ=255)
cgcCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTgcg < 2:346044/139‑1 (MQ=255)
cgcCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTgcg < 2:1817836/139‑1 (MQ=255)
ccGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAAt > 1:1200252/1‑139 (MQ=255)
gCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATag > 1:257275/1‑139 (MQ=255)
cGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGTAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATagcgag < 2:1494902/139‑1 (MQ=255)
gCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGtt > 1:574071/1‑98 (MQ=255)
gCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGtt < 2:574071/98‑1 (MQ=255)
gCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTCGTAGATAAACCCACCGCGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAACTagcgcgcg > 1:1412627/1‑139 (MQ=255)
ggTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAAc < 2:345348/139‑1 (MQ=255)
ggTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAAc < 2:717753/139‑1 (MQ=255)
aGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAacacac > 1:375865/1‑73 (MQ=255)
aGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAacacac < 2:375865/73‑1 (MQ=255)
tgtTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATagcgag < 2:1391434/117‑1 (MQ=255)
tgtTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATagcgag > 1:1391434/1‑117 (MQ=255)
aaCGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCa < 2:1200252/139‑1 (MQ=255)
cGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAgg > 2:715091/1‑127 (MQ=255)
cGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAgg < 1:715091/127‑1 (MQ=255)
cGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATc > 2:653605/1‑139 (MQ=255)
tACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGtt > 2:560794/1‑55 (MQ=255)
tACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGtt < 1:560794/55‑1 (MQ=255)
cggcggCGACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATcgccgc > 1:825432/1‑139 (MQ=255)
gcgACCGGAATACCGATACTGTTGTAGATAAAcaca < 2:1505912/36‑1 (MQ=255)
gcgACCGGAATACCGATACTGTTGTAGATAAAcaca > 1:1505912/1‑36 (MQ=255)
gcgACCGGAATACCGATACTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGttt > 1:1901738/1‑139 (MQ=255)
cTGTTGTAGATAAACACACCGAGCGGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTgc > 1:1391371/1‑139 (MQ=255)
cTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTtgtg > 2:959386/1‑44 (MQ=255)
cTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTtgtg < 1:959386/44‑1 (MQ=255)
cTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTgc > 2:311598/1‑139 (MQ=255)
cTGTTGTAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTgc > 1:1414626/1‑139 (MQ=255)
ttgtAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTa > 2:1845222/1‑90 (MQ=255)
ttgtAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTa < 1:1845222/90‑1 (MQ=255)
ttgtAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATg < 1:905064/92‑1 (MQ=255)
ttgtAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATg > 2:905064/1‑92 (MQ=255)
ttgtAGATAAACACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTgccgc > 1:1976820/1‑139 (MQ=255)
aaCACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTGCCTCCACCCATCg < 1:311598/139‑1 (MQ=255)
aaCACACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTGCCGCCACCCATCg < 2:1976820/139‑1 (MQ=255)
|
CCGGTTGGCGTTACTCACTACGGTAATCGACGAGAGCGCCATTGCCGCTCCGGCAACTACCGGGTTAAGCAGTGTTCCAGTGAACGGCCACAAAATACCGGCGGCGACCGGAATACCGATACTGTTGTAGATAAACGCACCGAGCAGGTTCTGCTTCATGTTGTGCAGCGTTGCGCGGGAAATAGCGAGCGCATCCGCAACGCCCATCAGGCTATGGCGCATCAGGGTAATCGCCGCGGTTTCAATGGCAACATCACTGCCGCCACCCATCG > NC_000913/508902‑509173
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
