Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 773 79.9 4112934 92.2% 3792125 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 892,869 G→A *301* (TAG→TAA)  rimK → ribosomal protein S6 modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913892,8690GA100.0% 229.8 / NA 70*301* (TAG→TAA) rimKribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/36);  total (34/36)

GTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAA  >  NC_000913/892738‑892998
                                                                                                                                   |                                                                                                                                 
gTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            <  1:463969/139‑1 (MQ=255)
gTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            <  2:756711/139‑1 (MQ=255)
         gTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACt                                                                                                                   <  1:727524/139‑1 (MQ=255)
           gAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGa                                                                                                                 >  2:1384838/1‑139 (MQ=255)
                 gTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                             >  2:1570507/1‑121 (MQ=255)
                 gTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                             <  1:1570507/121‑1 (MQ=255)
                  tCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            >  2:527035/1‑121 (MQ=255)
                  tCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            <  1:527035/121‑1 (MQ=255)
                  tCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                     >  2:1993878/1‑128 (MQ=255)
                  tCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                     <  1:1993878/128‑1 (MQ=255)
                    gCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACAtt                                                                                                                      >  2:1858777/1‑125 (MQ=255)
                    gCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACAtt                                                                                                                      <  1:1858777/125‑1 (MQ=255)
                        gggCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGcc                                                                                                    >  2:426061/1‑139 (MQ=255)
                                   aaTAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               <  2:1377151/101‑1 (MQ=255)
                                   aaTAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               >  1:1377151/1‑101 (MQ=255)
                                     tAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAAAGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCg                                                                                       <  1:789119/139‑1 (MQ=255)
                                        aaaaaaCCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTaa                                                                                    >  2:383723/1‑139 (MQ=255)
                                         aaaaaCCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAg                                                                                   <  2:7344/139‑1 (MQ=255)
                                             accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTgcg                                                                                                >  2:1267313/1‑122 (MQ=255)
                                             accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTgcg                                                                                                <  1:1267313/122‑1 (MQ=255)
                                             accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCtgt                                                                               >  2:1546144/1‑139 (MQ=255)
                                             accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCtgt                                                                               >  2:1732627/1‑139 (MQ=255)
                                               caccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGc                                                                             <  1:1732627/139‑1 (MQ=255)
                                                 ccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCg                                                                           <  1:1384838/139‑1 (MQ=255)
                                                     tATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCt                                                                       >  2:843300/1‑139 (MQ=255)
                                                          aCATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                     <  1:287253/88‑1 (MQ=255)
                                                          aCATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                     >  2:287253/1‑88 (MQ=255)
                                                          aCATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTa                                                                  >  1:622509/1‑139 (MQ=255)
                                                            aTCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            >  2:1608043/1‑79 (MQ=255)
                                                            aTCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            <  1:1608043/79‑1 (MQ=255)
                                                              cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGa                                                              >  1:250723/1‑139 (MQ=255)
                                                               gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               >  2:1114358/1‑73 (MQ=255)
                                                               gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               <  1:1114358/73‑1 (MQ=255)
                                                                 gggTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAag                                                           <  1:1785086/139‑1 (MQ=255)
                                                                        aTGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGt                                                    <  2:622509/139‑1 (MQ=255)
                                                                         tGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTa                                                   <  1:843300/139‑1 (MQ=255)
                                                                          gATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                  <  1:361775/139‑1 (MQ=255)
                                                                             ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            <  1:1735792/62‑1 (MQ=255)
                                                                             ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            >  2:1735792/1‑62 (MQ=255)
                                                                                cTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAAtt                                            >  2:1687925/1‑139 (MQ=255)
                                                                                   gATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATg                                         >  1:1322989/1‑139 (MQ=255)
                                                                                    aTCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGa                                        >  1:989705/1‑139 (MQ=255)
                                                                                       gAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACAt                                     <  1:383723/139‑1 (MQ=255)
                                                                                        aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                               <  1:2049753/64‑1 (MQ=255)
                                                                                        aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                               >  2:2049753/1‑64 (MQ=255)
                                                                                        aaCCCCACGCTACGACAGAAGATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGAACAAAATTATGACATc                                    >  2:1396497/1‑139 (MQ=255)
                                                                                           gCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                               >  1:2049926/1‑61 (MQ=255)
                                                                                           gCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                               <  2:2049926/61‑1 (MQ=255)
                                                                                             cACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTgg                               >  1:496272/1‑139 (MQ=255)
                                                                                                          aaTATTGCCTGAGAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                  <  1:607307/107‑1 (MQ=255)
                                                                                                          aaTATTGCCTGAGAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                  >  2:607307/1‑107 (MQ=255)
                                                                                                          aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTcc                        >  2:621975/1‑133 (MQ=255)
                                                                                                          aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTcc                        <  1:621975/133‑1 (MQ=255)
                                                                                                            tatTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAg                                                                                   >  1:536945/1‑72 (MQ=255)
                                                                                                            tatTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAg                                                                                   <  2:536945/72‑1 (MQ=255)
                                                                                                            tatTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGaa                                                             >  2:173663/1‑94 (MQ=255)
                                                                                                            tatTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGaa                                                             <  1:173663/94‑1 (MQ=255)
                                                                                                                gCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATAc            >  1:262198/1‑139 (MQ=255)
                                                                                                                gCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATAc            <  2:989705/139‑1 (MQ=255)
                                                                                                                 ccTGATAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                              <  2:1152280/120‑1 (MQ=255)
                                                                                                                 ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                              >  1:1152280/1‑120 (MQ=255)
                                                                                                                    gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCt                                                                                              >  1:985298/1‑53 (MQ=255)
                                                                                                                    gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCt                                                                                              <  2:985298/53‑1 (MQ=255)
                                                                                                                     aaaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGc       <  2:262198/139‑1 (MQ=255)
                                                                                                                       aaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGt     <  1:1687925/139‑1 (MQ=255)
                                                                                                                          gggTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCaa  <  1:1222406/139‑1 (MQ=255)
                                                                                                                           ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                            >  2:2039067/1‑64 (MQ=255)
                                                                                                                           ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                            <  1:2039067/64‑1 (MQ=255)
                                                                                                                           ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCtttt                                                                    <  2:1436215/72‑1 (MQ=255)
                                                                                                                           ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCtttt                                                                    >  1:1436215/1‑72 (MQ=255)
                                                                                                                                   |                                                                                                                                 
GTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAA  >  NC_000913/892738‑892998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A