Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 773 79.9 4112934 92.2% 3792125 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,268,531 G→A G235R (GGG→AGG)  yeiR → Zn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,268,5310GA100.0% 190.1 / NA 58G235R (GGG→AGG) yeiRZn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/31);  total (27/31)

TTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCG  >  NC_000913/2268397‑2268654
                                                                                                                                      |                                                                                                                           
ttctGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCa                                                                                                                         <  2:1757240/139‑1 (MQ=255)
   tGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAgg                                                                                                                      >  2:448100/1‑139 (MQ=255)
       tttGCCGCGTCGCAATTAAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGatat                                                                                                                  >  2:1287183/1‑139 (MQ=255)
          gCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAg                                                                                                               >  2:1017743/1‑139 (MQ=255)
          gCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAg                                                                                                               >  1:892537/1‑139 (MQ=255)
          gCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAg                                                                                                               >  2:388977/1‑139 (MQ=255)
                           ccGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATAtt                                                                                              >  2:1756834/1‑139 (MQ=255)
                           ccGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATAtt                                                                                              <  2:892537/139‑1 (MQ=255)
                              aGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGa                                                                                           <  2:9944/139‑1 (MQ=255)
                                ttGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCaa                                                                                                                        <  2:904862/108‑1 (MQ=255)
                                ttGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCaa                                                                                                                        >  1:904862/1‑108 (MQ=255)
                                ttGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACg                                                                                         <  2:235115/139‑1 (MQ=255)
                                      gCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATa                                                                                   <  2:796943/139‑1 (MQ=255)
                                          gcgcCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATa                                                                                   <  1:2008885/135‑1 (MQ=255)
                                          gcgcCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATa                                                                                   >  2:2008885/1‑135 (MQ=255)
                                                        catcaGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg                                                                 <  1:1756834/139‑1 (MQ=255)
                                                        catcaGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg                                                                 <  1:448100/139‑1 (MQ=255)
                                                          tcaGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGc                                                               <  1:1287183/139‑1 (MQ=255)
                                                          tcaGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGc                                                               <  1:286704/139‑1 (MQ=255)
                                                            aGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGac                                                                        >  2:491656/1‑128 (MQ=255)
                                                            aGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGac                                                                        <  1:491656/128‑1 (MQ=255)
                                                                 gTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATa                                                                                   >  2:2023810/1‑112 (MQ=255)
                                                                 gTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATa                                                                                   <  1:2023810/112‑1 (MQ=255)
                                                                 gTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg                                                                 <  1:405786/130‑1 (MQ=255)
                                                                 gTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg                                                                 >  2:405786/1‑130 (MQ=255)
                                                                 gTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTgg                                                        >  2:1041102/1‑139 (MQ=255)
                                                                   cGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCa                                                                                                                         <  1:294567/72‑1 (MQ=255)
                                                                   cGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCa                                                                                                                         >  2:294567/1‑72 (MQ=255)
                                                                     tGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg                                                                 <  1:337293/126‑1 (MQ=255)
                                                                     tGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg                                                                 >  2:337293/1‑126 (MQ=255)
                                                                        aaaaaGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGgc                                                 <  2:1139179/139‑1 (MQ=255)
                                                                        aaaaaGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGgc                                                 >  1:1648778/1‑139 (MQ=255)
                                                                         aaaaGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGgcg                                                <  1:1156747/139‑1 (MQ=255)
                                                                           aaGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGcgcg                                              >  2:1695561/1‑139 (MQ=255)
                                                                            aGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTg                                                                                      >  1:1910957/1‑98 (MQ=255)
                                                                            aGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTg                                                                                      <  2:1910957/98‑1 (MQ=255)
                                                                            aGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg                                                                 <  1:1055893/119‑1 (MQ=255)
                                                                            aGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg                                                                 >  2:1055893/1‑119 (MQ=255)
                                                                                ttAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTg                                         <  1:1041102/139‑1 (MQ=255)
                                                                                  agcagcGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCa                                       >  1:161331/1‑139 (MQ=255)
                                                                                         ttAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTgg                                >  2:1723531/1‑139 (MQ=255)
                                                                                           aaGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGaa                              <  2:1648778/139‑1 (MQ=255)
                                                                                           aaGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGaa                              <  2:161331/139‑1 (MQ=255)
                                                                                                      agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGcc                                                                                                            <  2:194087/50‑1 (MQ=255)
                                                                                                      agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGcc                                                                                                            >  1:194087/1‑50 (MQ=255)
                                                                                                      agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa                                                                         >  2:1698882/1‑85 (MQ=255)
                                                                                                      agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa                                                                         <  1:1698882/85‑1 (MQ=255)
                                                                                                        cATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCa                                                                                                                         >  2:603788/1‑35 (MQ=255)
                                                                                                        cATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCa                                                                                                                         <  1:603788/35‑1 (MQ=255)
                                                                                                          tCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAAGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa                                                                         >  2:1345602/1‑81 (MQ=255)
                                                                                                          tCAACGCTGGCGTCGCAGACTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGATATTCGa                                                                         <  1:1345602/81‑1 (MQ=255)
                                                                                                           cAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGa                                                                                           <  2:1190849/62‑1 (MQ=255)
                                                                                                           cAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTAGATATTCGa                                                                                           >  1:1190849/1‑62 (MQ=255)
                                                                                                               gCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCg          <  2:1341029/139‑1 (MQ=255)
                                                                                                                 tGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTa        >  2:1801901/1‑139 (MQ=255)
                                                                                                                  ggCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTAt       >  1:1784986/1‑139 (MQ=255)
                                                                                                                  ggCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTAt       <  1:1695561/139‑1 (MQ=255)
                                                                                                                       cGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCg  <  1:1801901/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                           
TTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCG  >  NC_000913/2268397‑2268654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A