Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,268,531 |
G→A |
G235R (GGG→AGG) |
yeiR → |
Zn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,268,531 | 0 | G | A | 100.0%
| 112.5
/ NA
| 35 | G235R (GGG→AGG) | yeiR | Zn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/19); total (16/19) |
TCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCT > NC_000913/2268395‑2268643
|
tcttctGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCagg > 1:726740/1‑139 (MQ=255)
tcttctGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCagg > 1:863228/1‑139 (MQ=255)
tGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCa > 2:625214/1‑139 (MQ=255)
tGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCa > 1:297165/1‑139 (MQ=255)
tGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCa > 2:142287/1‑139 (MQ=255)
gCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAg > 2:460470/1‑139 (MQ=255)
gcgTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGcc < 1:286686/139‑1 (MQ=255)
cGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCg < 1:917172/139‑1 (MQ=255)
ccGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATAtt < 1:313711/139‑1 (MQ=255)
ttGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTc > 2:548222/1‑135 (MQ=255)
ttGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTc < 1:548222/135‑1 (MQ=255)
ttGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACg < 1:625214/139‑1 (MQ=255)
gCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTc > 1:30661/1‑133 (MQ=255)
gCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTc < 2:30661/133‑1 (MQ=255)
gCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATa < 1:142287/139‑1 (MQ=255)
cAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACg < 1:1111641/139‑1 (MQ=255)
catcaGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg < 2:726740/139‑1 (MQ=255)
gTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTgg > 1:1030621/1‑139 (MQ=255)
aaaaaGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGc > 2:946499/1‑100 (MQ=255)
aaaaaGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGc < 1:946499/100‑1 (MQ=255)
gTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCagg < 2:579408/49‑1 (MQ=255)
gTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCagg > 1:579408/1‑49 (MQ=255)
gTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTg > 1:885587/1‑139 (MQ=255)
aGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAAc > 2:1000841/1‑139 (MQ=255)
gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACg > 1:678695/1‑82 (MQ=255)
gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACg < 2:678695/82‑1 (MQ=255)
gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTg < 1:739519/98‑1 (MQ=255)
gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTc > 1:397543/1‑139 (MQ=255)
gCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGCATATTCGACGCTGATACGGTATTCGACACCATTg > 2:739519/1‑98 (MQ=255)
agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAATGATATCAGGCCTGCGGCTGGATATTCGa < 1:703450/67‑1 (MQ=255)
agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGa > 2:703450/1‑67 (MQ=255)
agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa > 2:582989/1‑85 (MQ=255)
agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa < 1:582989/85‑1 (MQ=255)
agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa > 2:808251/1‑85 (MQ=255)
agCATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGa < 1:808251/85‑1 (MQ=255)
cATCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCg < 2:885587/139‑1 (MQ=255)
tCAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGtg < 2:863228/139‑1 (MQ=255)
cAACGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGtgc < 2:397543/139‑1 (MQ=255)
aaCGCTGGCGTCGCAGTCTGAACAGCAGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGtgct < 2:297165/139‑1 (MQ=255)
|
TCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCT > NC_000913/2268395‑2268643
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
