Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,867,382 |
G→T |
A57E (GCG→GAG) |
rpoS ← |
RNA polymerase, sigma S (sigma 38) factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,867,382 | 0 | G | T | 100.0%
| 131.0
/ NA
| 36 | A57E (GCG→GAG) | rpoS | RNA polymerase, sigma S (sigma 38) factor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (15/21); total (15/21) |
CGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCT > NC_000913/2867305‑2867459
|
cgcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGt > 2:517143/1‑81 (MQ=255)
cgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTcc < 2:517100/81‑1 (MQ=255)
gACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCa < 1:517101/81‑1 (MQ=255)
aCGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCaa < 1:517103/81‑1 (MQ=255)
aCGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCaa < 2:517102/81‑1 (MQ=255)
tAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCtgt < 1:517104/81‑1 (MQ=255)
aaCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCtgtgt < 1:517105/81‑1 (MQ=255)
tcttcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTccc > 1:517155/1‑81 (MQ=255)
cttcGGCCGTTATCAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTccc < 1:517106/80‑1 (MQ=255)
cttcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCt > 2:517160/1‑81 (MQ=255)
cGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCt < 1:517107/78‑1 (MQ=255)
ggCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGa > 1:517166/1‑81 (MQ=255)
ccGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATa < 1:517108/81‑1 (MQ=255)
cGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATaa < 2:517109/81‑1 (MQ=255)
gTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTTCAACACACGCTGTGTGGCTCCCTGCGATaa > 2:517167/1‑80 (MQ=255)
gTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATaa > 2:517148/1‑80 (MQ=255)
gTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATaa > 1:517150/1‑80 (MQ=255)
aCAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGtt < 2:517110/81‑1 (MQ=255)
aGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTcc > 1:517151/1‑81 (MQ=255)
tggtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCctc < 1:517111/81‑1 (MQ=255)
tgAATAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTc < 2:517112/81‑1 (MQ=255)
aaTAACCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCgg < 2:517113/81‑1 (MQ=255)
aaCCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGcc > 1:517164/1‑80 (MQ=255)
aCCAATCTCACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCa < 1:517114/80‑1 (MQ=255)
cACCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTAt > 2:517157/1‑81 (MQ=255)
aCCAAGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGtt < 2:517115/78‑1 (MQ=255)
aCCAAGGTAAAGATGAGTCTCATCCAGCACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGtt < 2:517116/78‑1 (MQ=255)
aGGTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTg > 2:517161/1‑81 (MQ=255)
gTAAAGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTggg > 1:517170/1‑81 (MQ=255)
aaaGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGtt > 1:517162/1‑81 (MQ=255)
aaGCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTc > 1:517174/1‑81 (MQ=255)
gCTGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCt < 2:517117/81‑1 (MQ=255)
tGAGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGt > 1:517156/1‑81 (MQ=255)
aGTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTc < 1:517118/81‑1 (MQ=255)
gTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTct < 2:517119/81‑1 (MQ=255)
gTCTCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTct < 2:517120/81‑1 (MQ=255)
|
CGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCT > NC_000913/2867305‑2867459
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
