Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.
ALE, Flask, Isolate, Technical Replicate | Clonal or Population | ALE Sample Details | Base Media | Carbon Source(g/L) | Nitrogen Source(g/L) | Phosphorus Source(g/L) | Sulfur Source(g/L) | Calcium Source(g/L) | Supplement(g/L) | Temperature (Celsius) | Strain | Additional Strain Details | Taxonomy ID | Reference Genome |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A0 F0 I1 R1 | clonal | ALE starts with 0.03 ug/mL gentamycin which is increased up to 256 g/mL during the ALE | M9 | Glucose(2 g/L) | NH4Cl(1) | KH2PO4(3),Na2HPO4(6.8) | MgSO4(0.24) | CaCl2(0.1) | gentamycin(0.03), | 37 | 511145 | E. coli K-12 MG1655 ΔdnaQ | /var/data/NC_000913.3.gb | |
A1 F1 I1 R1 | clonal | ALE starts with 0.03 ug/mL gentamycin which is increased up to 256 g/mL during the ALE | M9 | Glucose(2 g/L) | NH4Cl(1) | KH2PO4(3),Na2HPO4(6.8) | MgSO4(0.24) | CaCl2(0.1) | gentamycin(0.03), | 37 | 511145 | E. coli K-12 MG1655 ΔdnaQ | /var/data/NC_000913.3.gb | |
A1 F1 I2 R1 | clonal | ALE starts with 0.03 ug/mL gentamycin which is increased up to 256 g/mL during the ALE | M9 | Glucose(2 g/L) | NH4Cl(1) | KH2PO4(3),Na2HPO4(6.8) | MgSO4(0.24) | CaCl2(0.1) | gentamycin(0.03), | 37 | 511145 | E. coli K-12 MG1655 ΔdnaQ | /var/data/NC_000913.3.gb | |
A2 F1 I1 R1 | clonal | ALE starts with 0.03 ug/mL gentamycin which is increased up to 256 g/mL during the ALE | M9 | Glucose(2 g/L) | NH4Cl(1) | KH2PO4(3),Na2HPO4(6.8) | MgSO4(0.24) | CaCl2(0.1) | gentamycin(0.03), | 37 | 511145 | E. coli K-12 MG1655 ΔdnaQ | /var/data/NC_000913.3.gb | |
A3 F1 I1 R1 | clonal | ALE starts with 0.03 ug/mL gentamycin which is increased up to 256 g/mL during the ALE | M9 | Glucose(2 g/L) | NH4Cl(1) | KH2PO4(3),Na2HPO4(6.8) | MgSO4(0.24) | CaCl2(0.1) | gentamycin(0.03), | 37 | 511145 | E. coli K-12 MG1655 ΔdnaQ | /var/data/NC_000913.3.gb |