breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 65,122 A→C I220S (ATC→AGC)  polB ← DNA polymerase II
RA 79,341 C→A G38V (GGT→GTT)  leuD ← 3‑isopropylmalate dehydratase subunit LeuD
RA 91,323 G→A E98K (GAA→AAA)  ftsL → cell division protein FtsL
RA 104,426 C→T L149F (CTT→TTT)  ftsA → cell division protein FtsA
RA 111,731 T→C E39G (GAA→GGA)  yacG ← DNA gyrase inhibitor YacG
RA 164,352 C→T L750L (CTG→TTG)  hrpB → putative ATP‑dependent RNA helicase HrpB
RA 167,915 Δ1 bp coding (432/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
RA 181,536 C→G A218G (GCC→GGC)  degP → periplasmic serine endoprotease DegP
RA 191,485 A→G E210G (GAA→GGA)  tsf → protein chain elongation factor EF‑Ts
RA 208,192 T→A S1023T (TCC→ACC)  dnaE → DNA polymerase III subunit alpha
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 268,409 C→T E184E (GAG→GAA ykfA ← CP4‑6 prophage; putative GTP‑binding protein YkfA
RA 294,225 T→G N192T (AAC→ACC)  yagM ← CP4‑6 prophage; protein YagM
RA 304,754 C→T R151Q (CGA→CAA)  ecpE ← putative fimbrial chaperone EcpE
RA 311,801 G→A intergenic (‑465/‑311) ecpR ← / → ykgL DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL
RA 314,734 Δ1 bp pseudogene (378/888 nt) eaeH → putative porin domain‑containing protein EaeH
RA 334,105 T→G H110P (CAC→CCC)  yahB ← putative LysR‑type DNA‑binding transcriptional regulator YahB
RA 341,434 C→G P104A (CCG→GCG)  yahJ → putative deaminase with metallo‑dependent hydrolase domain
RA 353,323 C→G S206R (AGC→AGG prpE → propionyl‑CoA synthetase
RA 396,645 A→T K3* (AAG→TAG)  sbmA → peptide antibiotic/peptide nucleic acid transporter
RA 399,509 T→C intergenic (‑176/‑84) yaiY ← / → yaiZ inner membrane protein/DUF2754 domain‑containing protein YaiZ
RA 403,036 T→C D430D (GAT→GAC phoA → alkaline phosphatase
RA 432,294 A→G intergenic (‑281/+18) tsx ← / ← yajI nucleoside‑specific channel‑forming protein Tsx/putative lipoprotein YajI
RA 449,803 C→A W280L (TGG→TTG)  cyoB ← cytochrome bo3 ubiquinol oxidase subunit 1
RA 461,034 T→G I716S (ATC→AGC)  lon → Lon protease
RA 508,288 A→G D24G (GAT→GGT)  ybaQ → putative DNA‑binding transcriptional regulator YbaQ
RA 538,029 A→G I133V (ATT→GTT)  ybbW → putative allantoin transporter
RA 612,670 A→G intergenic (‑176/‑67) fepA ← / → fes ferric enterobactin outer membrane transporter/enterochelin esterase
RA 656,725 T→A W57R (TGG→AGG)  pagP → Lipid IVA palmitoyltransferase
RA 698,541 G→T R213S (CGT→AGT)  asnB ← asparagine synthetase B
RA 797,736 T→C E232E (GAA→GAG ybhA ← pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
RA 814,742 G→C G406A (GGC→GCC)  uvrB → excision nuclease subunit B
RA 909,605 A→T L482Q (CTA→CAA)  poxB ← pyruvate oxidase
RA 913,852 Δ1 bp intergenic (‑38/+106) hcp ← / ← lysO protein S‑nitrosylase/L‑lysine exporter
RA 914,159 T→C E233E (GAA→GAG lysO ← L‑lysine exporter
RA 919,229 A→G intergenic (‑109/‑6) ybjX ← / → macA DUF535 domain‑containing protein YbjX/ABC‑type tripartite efflux pump membrane fusion protein
RA 922,079 T→G L578R (CTG→CGG)  macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 996,703 C→G G81A (GGC→GCC)  ssuA ← aliphatic sulfonate ABC transporter periplasmic binding protein
RA 1,007,421 T→C S60S (TCA→TCG ycbX ← 6‑N‑hydroxylaminopurine resistance protein
RA 1,026,163 A→C I565L (ATC→CTC)  helD → DNA helicase IV
RA 1,042,554 C→G A553P (GCA→CCA)  etk ← protein‑tyrosine kinase Etk
RA 1,064,853 A→T D273V (GAT→GTT)  yccE → uncharacterized protein YccE
RA 1,096,234 C→G P131R (CCG→CGG)  ycdU → uncharacterized protein YcdU
RA 1,104,203 A→G T85A (ACA→GCA)  csgB → curlin, minor subunit
RA 1,143,915 T→A L151F (TTA→TTT rne ← ribonuclease E
RA 1,182,052 C→G A260P (GCG→CCG)  potD ← spermidine preferential ABC transporter periplasmic binding protein
RA 1,198,448 G→A intergenic (‑211/‑247) ymfE ← / → lit e14 prophage; uncharacterized protein YmfE/e14 prophage; cell death peptidase Lit
RA 1,224,226 (A)5→4 intergenic (+319/+53) ycgI → / ← minE protein YcgI/Z‑ring positioning protein MinE
RA 1,277,223 G→C A132A (GCC→GCG narX ← sensory histidine kinase NarX
RA 1,281,926 T→A L688Q (CTG→CAG)  narG → nitrate reductase A subunit alpha
RA 1,284,681 T→C S360P (TCT→CCT)  narH → nitrate reductase A subunit beta
RA 1,293,171 G→A intergenic (‑249/‑356) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
RA 1,299,534 T→C F2L (TTT→CTT)  insH21 → IS5 transposase and trans‑activator
RA 1,307,693 G→A P318L (CCG→CTG)  clsA ← cardiolipin synthase A
RA 1,349,230 T→G K293T (AAA→ACA)  yciW ← putative oxidoreductase
RA 1,359,068 T→C V40V (GTA→GTG puuP ← putrescine:H(+) symporter PuuP
RA 1,428,030 C→G A233P (GCC→CCC)  insH‑5 ← Rac prophage; IS5 transposase and trans‑activator
RA 1,435,732 (A)5→4 intergenic (‑113/+28) uspF ← / ← ompN nucleotide binding filament protein/outer membrane porin N
RA 1,442,102 (T)5→4 coding (742/990 nt) ldhA ← D‑lactate dehydrogenase
RA 1,442,546 A→C Y100D (TAT→GAT)  ldhA ← D‑lactate dehydrogenase
RA 1,472,567 C→G pseudogene (2049/3495 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,490,954 (T)5→4 coding (53/531 nt) cybB → cytochrome b561
RA 1,497,760 A→C D302A (GAT→GCT)  opgD → glucan biosynthesis protein D
RA 1,506,320 C→A A50E (GCG→GAG)  sutR → DNA‑binding transcriptional dual regulator SutR
RA 1,512,246 T→G V198G (GTT→GGT)  ydcS → putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
RA 1,520,982 A→G I695T (ATT→ACT)  yncD ← putative TonB‑dependent outer membrane receptor
RA 1,562,457 G→A P9S (CCC→TCC)  ddpA ← putative D,D‑dipeptide ABC transporter periplasmic binding protein
RA 1,572,565 T→C T880A (ACT→GCT)  pqqL ← putative zinc peptidase
RA 1,575,336 T→G S762R (AGC→CGC)  yddB ← putative TonB‑dependent receptor
RA 1,578,871 A→G F158L (TTC→CTC)  yddA ← ABC transporter family protein YddA
RA 1,598,270 A→T intergenic (‑184/+347) yneO ← / ← lsrK AIDA‑I family autotransporter YneO/autoinducer‑2 kinase
RA 1,607,828 C→T G161G (GGC→GGT tam → trans‑aconitate 2‑methyltransferase
RA 1,609,077 C→T intergenic (‑55/+152) yneE ← / ← uxaB conserved inner membrane protein YneE/tagaturonate reductase
RA 1,610,589 A→G V31A (GTT→GCT)  uxaB ← tagaturonate reductase
RA 1,650,406 C→T pseudogene (329/921 nt) ydfE → Qin prophage; protein YdfE
RA 1,658,706 G→C G213A (GGC→GCC)  ynfE → putative selenate reductase YnfE
RA 1,680,663 C→T P369P (CCC→CCT ydgI → putative arginine:ornithine antiporter
RA 1,693,155 Δ1 bp coding (1109/1374 nt) uidB ← glucuronide:H(+) symporter
RA 1,706,464 A→T K39N (AAA→AAT rsxB → SoxR [2Fe‑2S] reducing system protein RsxB
RA 1,750,974 T→A T14S (ACA→TCA)  ydhX ← putative 4Fe‑4S ferredoxin‑type protein
RA 1,774,321 T→C V178A (GTC→GCC)  ydiB → shikimate dehydrogenase/quinate dehydrogenase
RA 1,779,461 G→C intergenic (‑160/‑156) ydiP ← / → ydiQ putative DNA‑binding transcriptional regulator YdiP/putative electron transfer flavoprotein subunit YdiQ
RA 1,796,866 T→C Y360C (TAT→TGT)  pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,799,179 Δ1 bp intergenic (‑237/+47) pheS ← / ← pheM phenylalanine‑‑tRNA ligase subunit alpha/pheST‑ihfA operon leader peptide
RA 1,830,943 T→C Y347C (TAC→TGC)  astC ← succinylornithine transaminase
RA 1,832,618 G→C G64A (GGC→GCC)  xthA → exodeoxyribonuclease III
RA 1,899,060 A→C N212H (AAT→CAT)  pdeD → putative c‑di‑GMP phosphodiesterase PdeD
RA 1,915,177 (T)6→5 coding (358/699 nt) proQ ← RNA chaperone ProQ
RA 1,928,777 A→G D21D (GAT→GAC ptrB ← oligopeptidase B
RA 1,932,077 T→G intergenic (+18/+38) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase
RA 1,967,269 C→A D57Y (GAC→TAC)  cheY ← chemotaxis protein CheY
RA 1,977,712 T→C K45E (AAA→GAA)  flhC ← DNA‑binding transcriptional dual regulator FlhC
RA 1,988,032 T→C intergenic (‑73/‑190) azuC ← / → yecR uncharacterized protein AzuC/lipoprotein YecR
RA 1,990,885 T→A V402E (GTG→GAG)  tyrP → tyrosine:H(+) symporter
RA 1,995,681 T→C Y9Y (TAT→TAC yecU → protein YecU
RA 1,995,748 C→G L32V (CTT→GTT)  yecU → protein YecU
RA 2,014,078 G→A D284N (GAT→AAT)  fliF → flagellar basal‑body MS‑ring and collar protein
RA 2,015,409 A→G E177G (GAA→GGA)  fliG → flagellar motor switch protein FliG
RA 2,044,771 Δ1 bp intergenic (+147/‑167) asnT → / → yeeJ tRNA‑Asn/inverse autotransporter adhesin
RA 2,062,025 T→C intergenic (‑92/‑235) nac ← / → asnV DNA‑binding transcriptional dual regulator Nac/tRNA‑Asn
RA 2,075,218 C→A A147E (GCG→GAG)  yeeR → CP4‑44 prophage; inner membrane protein YeeR
RA 2,107,517 A→T D271E (GAT→GAA glf ← UDP‑galactopyranose mutase
RA 2,113,482 T→C K282K (AAA→AAG galF ← UTP:glucose‑1‑phosphate uridylyltransferase, low activity
RA 2,140,712 C→A E301* (GAA→TAA)  asmA ← putative assembly protein AsmA
RA 2,150,632 Δ1 bp coding (354/1947 nt) yegI ← protein kinase YegI
RA 2,183,270 C→A A128S (GCG→TCG)  yegX ← putative hydrolase
RA 2,221,701 T→C M97V (ATG→GTG)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,249,633 T→C intergenic (‑15/‑84) yeiE ← / → yeiH putative LysR‑type transcriptional regulator YeiE/conserved inner membrane protein YeiH
RA 2,259,134 T→A N55Y (AAC→TAC)  psuK ← putative pseudouridine kinase
RA 2,260,779 T→C K211R (AAA→AGA)  fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,275,772 A→G Y167C (TAT→TGT)  yejE → putative oligopeptide ABC transporter membrane subunit YejE
RA 2,451,016 A→G D20D (GAT→GAC yfcQ ← putative fimbrial protein YfcQ
RA 2,513,154 T→C V38A (GTT→GCT)  nupC → nucleoside:H(+) symporter NupC
RA 2,517,367 (A)5→4 coding (466/2190 nt) pdeA ← putative c‑di‑GMP phosphodiesterase PdeA
RA 2,660,768 G→C A255G (GCC→GGC)  iscS ← cysteine desulfurase
RA 2,692,082 C→G W1154C (TGG→TGC purL ← phosphoribosylformylglycinamide synthetase
RA 2,693,373 G→C A724G (GCC→GGC)  purL ← phosphoribosylformylglycinamide synthetase
RA 2,716,500 A→G intergenic (‑51/‑254) grcA ← / → ung stress‑induced alternate pyruvate formate‑lyase subunit/uracil‑DNA glycosylase
RA 2,717,817 C→T A238T (GCG→ACG)  yfiF ← putative methyltransferase
JC JC 2,737,118 IS1 (+) +8 bp intergenic (+235/‑29) bamD → / → raiA outer membrane protein assembly factor BamD/stationary phase translation inhibitor and ribosome stability factor
RA 2,783,901 C→T V177I (GTT→ATT)  ypjB ← DUF5508 domain‑containing protein YpjB
RA 2,810,778 T→C S3S (AGT→AGC mprA → DNA‑binding transcriptional repressor MprA
RA 2,887,571 Δ1 bp intergenic (+163/+7) sokX → / ← cysH small RNA SokX/phosphoadenosine phosphosulfate reductase
RA 2,889,110 T→C K330K (AAA→AAG cysI ← sulfite reductase, hemoprotein subunit
RA 2,907,282 A→G A220A (GCT→GCC eno ← enolase
RA 2,994,461 (T)8→7 pseudogene (443/444 nt) ygeK ← putative DNA‑binding transcriptional regulator YgeK
RA 2,995,368 T→G pseudogene (653/707 nt) ygeN ← putative type III secretion system protein
RA 3,173,177 T→A P12P (CCT→CCA ygiN → putative quinol monooxygenase YgiN
RA 3,184,175 T→C T98A (ACC→GCC)  ribB ← 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,184,476 (A)7→8 intergenic (‑10/‑364) ribB ← / → ubiK 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/ubiquinone biosynthesis accessory factor UbiK
RA 3,189,625 C→A pseudogene (2194/2434 nt) yqiG → putative outer membrane usher protein YqiG
RA 3,190,185 T→C L102P (CTT→CCT)  yqiH → putative fimbrial chaperone YqiH
RA 3,206,410 T→A intergenic (‑154/‑53) ttdR ← / → ttdA DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
RA 3,216,290 G→C P68A (CCA→GCA)  nfeF ← NADPH‑dependent ferric chelate reductase
RA 3,243,047 A→T Y90N (TAT→AAT)  uxaA ← D‑altronate dehydratase
RA 3,267,297 A→C intergenic (‑232/‑83) tdcA ← / → tdcR DNA‑binding transcriptional activator TdcA/DNA‑binding transcriptional activator TdcR
RA 3,300,767 T→G V170V (GTA→GTC yhbT ← SCP2 domain‑containing protein YhbT
RA 3,308,605 G→T T107K (ACG→AAG)  nlpI ← lipoprotein NlpI
RA 3,362,820 C→T T5M (ACG→ATG)  yhcD → putative fimbrial usher protein YhcD
RA 3,418,650 A→G intergenic (+39/‑392) yhdV → / → yhdW lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW
RA 3,433,348 G→A N71N (AAC→AAT smf ← protein Smf
RA 3,447,858 G→T R98S (CGT→AGT)  rplN ← 50S ribosomal subunit protein L14
RA 3,471,700 A→T F605L (TTT→TTA fusA ← elongation factor G
RA 3,571,253 A→C F23V (TTC→GTC)  glgX ← limit dextrin alpha‑1,6‑glucohydrolase
RA 3,651,128 (T)7→8 intergenic (+466/‑163) arsC → / → yhiS arsenate reductase/putative uncharacterized protein YhiS
RA 3,667,617 (A)6→5 intergenic (‑37/+174) gadA ← / ← ccp glutamate decarboxylase A/cytochrome c peroxidase
RA 3,765,827 Δ1 bp coding (3645/4134 nt) rhsA → rhs element protein RhsA
RA 3,795,757 G→C A277P (GCG→CCG)  waaF → ADP‑heptose‑‑LPS heptosyltransferase 2
RA 3,797,159 (A)6→5 coding (212/1260 nt) waaL → O‑antigen ligase
RA 3,839,590 G→A A135V (GCG→GTG)  nlpA ← lipoprotein‑28
RA 3,852,657 T→C Y43C (TAC→TGC)  ilvB ← acetohydroxy acid synthase I subunit IlvB
RA 3,863,817 A→T intergenic (‑214/‑82) glvC ← / → yidP putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP
RA 3,913,188 T→G I161L (ATC→CTC)  glmS ← L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
RA 3,917,491 T→A K259* (AAA→TAA)  atpG ← ATP synthase F1 complex subunit gamma
RA 3,937,676 A→T Q128L (CAA→CTA)  rbsK → ribokinase
RA 3,941,643 T→G intergenic (‑316/‑165) yieP ← / → rrsC putative transcriptional regulator YieP/16S ribosomal RNA
RA 3,941,650 A→G intergenic (‑323/‑158) yieP ← / → rrsC putative transcriptional regulator YieP/16S ribosomal RNA
RA 3,949,783 (T)7→6 coding (187/1521 nt) yifB ← putative magnesium chelatase YifB
RA 3,988,602 G→T V155V (GTC→GTA hemX ← PF04375 family protein HemX
RA 4,007,952 A→G K66E (AAA→GAA)  rhtC → L‑threonine exporter
RA 4,033,611 G→T G156V (GGT→GTT)  trkH → K(+) transporter TrkH
RA 4,098,551 A→G G272G (GGA→GGG rhaS → DNA‑binding transcriptional activator RhaS
RA 4,104,284 G→T A231E (GCG→GAG)  cpxA ← sensory histidine kinase CpxA
RA 4,109,886 T→C intergenic (+63/‑44) sbp → / → cdh sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp/CDP‑diacylglycerol diphosphatase
RA 4,118,141 Δ1 bp intergenic (‑51/‑374) glpF ← / → zapB glycerol facilitator/cell division factor ZapB
RA 4,175,484 G→T noncoding (13/85 nt) tyrU → tRNA‑Tyr
RA 4,175,853 (T)6→5 intergenic (+24/‑91) thrT → / → tufB tRNA‑Thr/translation elongation factor Tu 2
RA 4,184,579 T→G I1112S (ATC→AGC)  rpoB → RNA polymerase subunit beta
RA 4,235,966 Δ1 bp coding (61/243 nt) yjbE → uncharacterized protein YjbE
RA 4,244,695 Δ1 bp coding (381/1545 nt) malF ← maltose ABC transporter membrane subunit MalF
RA 4,282,453 T→C N208S (AAT→AGT)  yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,299,439 T→C intergenic (‑73/+125) fdhF ← / ← mdtP formate dehydrogenase H/putative multidrug efflux pump outer membrane channel
RA 4,313,118 C→T intergenic (‑127/‑232) alsR ← / → rpiB DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
RA 4,371,109 G→A V29I (GTT→ATT)  groL → chaperonin GroEL
RA 4,389,355 T→A T6S (ACT→TCT)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,471,278 T→A D57V (GAT→GTT)  pyrI ← aspartate carbamoyltransferase, PyrI subunit
RA 4,503,704 A→G intergenic (‑273/‑354) insG ← / → yjhB KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter
RA 4,546,846 (A)5→4 coding (1751/2637 nt) fimD → type I fimbriae usher protein
RA 4,549,087 G→T G94W (GGG→TGG)  fimH → type 1 fimbriae D‑mannose specific adhesin
RA 4,584,768 A→C V665G (GTT→GGT)  hsdR ← type I restriction enzyme EcoKI endonuclease component
RA 4,638,175 A→G intergenic (+55/‑3) creC → / → creD sensory histidine kinase CreC/putative inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 236071 236784 714 3 [0] [2] 3 dnaQ DNA polymerase III subunit epsilon
* * ÷ NC_000913 1530067 1530148 82 3 [2] [2] 3 ydcD uncharacterized protein YdcD
* * ÷ NC_000913 2033870 2033914 45 3 [2] [2] 3 rseX/yedS small regulatory RNA RseX/putative outer membrane protein YedS, N‑terminal fragment
* * ÷ NC_000913 3423730–3424234 3424574–3424238 5–845 3 [2] [2] 3 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =3 (0.100)17 (0.710) 17/128 0.4 84.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 4 (0.170)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078053 (0.100)13 (0.540) 13/128 0.7 80.3% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 4 (0.170)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment