breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 38,036 G→T F360L (TTC→TTA caiB ← gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
RA 79,341 C→A G38V (GGT→GTT)  leuD ← 3‑isopropylmalate dehydratase subunit LeuD
RA 91,323 G→A E98K (GAA→AAA)  ftsL → cell division protein FtsL
RA 104,426 C→T L149F (CTT→TTT)  ftsA → cell division protein FtsA
RA 164,352 C→T L750L (CTG→TTG)  hrpB → putative ATP‑dependent RNA helicase HrpB
RA 191,485 A→G E210G (GAA→GGA)  tsf → protein chain elongation factor EF‑Ts
RA 208,192 T→A S1023T (TCC→ACC)  dnaE → DNA polymerase III subunit alpha
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 268,409 C→T E184E (GAG→GAA ykfA ← CP4‑6 prophage; putative GTP‑binding protein YkfA
RA 304,754 C→T R151Q (CGA→CAA)  ecpE ← putative fimbrial chaperone EcpE
RA 311,801 G→A intergenic (‑465/‑311) ecpR ← / → ykgL DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL
RA 314,734 Δ1 bp pseudogene (378/888 nt) eaeH → putative porin domain‑containing protein EaeH
RA 334,105 T→G H110P (CAC→CCC)  yahB ← putative LysR‑type DNA‑binding transcriptional regulator YahB
RA 341,434 C→G P104A (CCG→GCG)  yahJ → putative deaminase with metallo‑dependent hydrolase domain
RA 399,509 T→C intergenic (‑176/‑84) yaiY ← / → yaiZ inner membrane protein/DUF2754 domain‑containing protein YaiZ
RA 508,288 A→G D24G (GAT→GGT)  ybaQ → putative DNA‑binding transcriptional regulator YbaQ
RA 538,029 A→G I133V (ATT→GTT)  ybbW → putative allantoin transporter
RA 698,541 G→T R213S (CGT→AGT)  asnB ← asparagine synthetase B
RA 742,653 G→A intergenic (‑97/‑174) dtpD ← / → ybgI dipeptide:H(+) symporter DtpD/radiation resistance protein YbgI
RA 797,736 T→C E232E (GAA→GAG ybhA ← pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
RA 913,852 Δ1 bp intergenic (‑38/+106) hcp ← / ← lysO protein S‑nitrosylase/L‑lysine exporter
RA 914,159 T→C E233E (GAA→GAG lysO ← L‑lysine exporter
RA 919,229 A→G intergenic (‑109/‑6) ybjX ← / → macA DUF535 domain‑containing protein YbjX/ABC‑type tripartite efflux pump membrane fusion protein
RA 922,079 T→G L578R (CTG→CGG)  macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 1,064,853 A→T D273V (GAT→GTT)  yccE → uncharacterized protein YccE
RA 1,096,234 C→G P131R (CCG→CGG)  ycdU → uncharacterized protein YcdU
RA 1,104,203 A→G T85A (ACA→GCA)  csgB → curlin, minor subunit
RA 1,224,226 (A)5→4 intergenic (+319/+53) ycgI → / ← minE protein YcgI/Z‑ring positioning protein MinE
RA 1,277,223 G→C A132A (GCC→GCG narX ← sensory histidine kinase NarX
RA 1,284,681 T→C S360P (TCT→CCT)  narH → nitrate reductase A subunit beta
RA 1,299,534 T→C F2L (TTT→CTT)  insH21 → IS5 transposase and trans‑activator
RA 1,428,030 C→G A233P (GCC→CCC)  insH‑5 ← Rac prophage; IS5 transposase and trans‑activator
RA 1,435,732 (A)5→4 intergenic (‑113/+28) uspF ← / ← ompN nucleotide binding filament protein/outer membrane porin N
RA 1,490,954 (T)5→4 coding (53/531 nt) cybB → cytochrome b561
RA 1,497,760 A→C D302A (GAT→GCT)  opgD → glucan biosynthesis protein D
RA 1,512,246 T→G V198G (GTT→GGT)  ydcS → putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
RA 1,520,982 A→G I695T (ATT→ACT)  yncD ← putative TonB‑dependent outer membrane receptor
RA 1,575,336 T→G S762R (AGC→CGC)  yddB ← putative TonB‑dependent receptor
RA 1,578,871 A→G F158L (TTC→CTC)  yddA ← ABC transporter family protein YddA
RA 1,607,828 C→T G161G (GGC→GGT tam → trans‑aconitate 2‑methyltransferase
RA 1,610,589 A→G V31A (GTT→GCT)  uxaB ← tagaturonate reductase
RA 1,658,706 G→C G213A (GGC→GCC)  ynfE → putative selenate reductase YnfE
RA 1,680,663 C→T P369P (CCC→CCT ydgI → putative arginine:ornithine antiporter
RA 1,706,464 A→T K39N (AAA→AAT rsxB → SoxR [2Fe‑2S] reducing system protein RsxB
RA 1,750,974 T→A T14S (ACA→TCA)  ydhX ← putative 4Fe‑4S ferredoxin‑type protein
RA 1,774,321 T→C V178A (GTC→GCC)  ydiB → shikimate dehydrogenase/quinate dehydrogenase
RA 1,796,866 T→C Y360C (TAT→TGT)  pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,799,179 Δ1 bp intergenic (‑237/+47) pheS ← / ← pheM phenylalanine‑‑tRNA ligase subunit alpha/pheST‑ihfA operon leader peptide
RA 1,830,943 T→C Y347C (TAC→TGC)  astC ← succinylornithine transaminase
RA 1,832,618 G→C G64A (GGC→GCC)  xthA → exodeoxyribonuclease III
RA 1,915,177 (T)6→5 coding (358/699 nt) proQ ← RNA chaperone ProQ
RA 1,928,777 A→G D21D (GAT→GAC ptrB ← oligopeptidase B
RA 1,995,681 T→C Y9Y (TAT→TAC yecU → protein YecU
RA 1,995,748 C→G L32V (CTT→GTT)  yecU → protein YecU
RA 2,015,409 A→G E177G (GAA→GGA)  fliG → flagellar motor switch protein FliG
RA 2,044,771 Δ1 bp intergenic (+147/‑167) asnT → / → yeeJ tRNA‑Asn/inverse autotransporter adhesin
RA 2,113,482 T→C K282K (AAA→AAG galF ← UTP:glucose‑1‑phosphate uridylyltransferase, low activity
RA 2,150,632 Δ1 bp coding (354/1947 nt) yegI ← protein kinase YegI
RA 2,221,701 T→C M97V (ATG→GTG)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,259,134 T→A N55Y (AAC→TAC)  psuK ← putative pseudouridine kinase
RA 2,260,779 T→C K211R (AAA→AGA)  fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,451,016 A→G D20D (GAT→GAC yfcQ ← putative fimbrial protein YfcQ
RA 2,517,367 (A)5→4 coding (466/2190 nt) pdeA ← putative c‑di‑GMP phosphodiesterase PdeA
RA 2,693,373 G→C A724G (GCC→GGC)  purL ← phosphoribosylformylglycinamide synthetase
RA 2,717,817 C→T A238T (GCG→ACG)  yfiF ← putative methyltransferase
JC JC 2,737,118 IS1 (+) +8 bp intergenic (+235/‑29) bamD → / → raiA outer membrane protein assembly factor BamD/stationary phase translation inhibitor and ribosome stability factor
RA 2,887,571 Δ1 bp intergenic (+163/+7) sokX → / ← cysH small RNA SokX/phosphoadenosine phosphosulfate reductase
RA 2,889,110 T→C K330K (AAA→AAG cysI ← sulfite reductase, hemoprotein subunit
RA 3,189,625 C→A pseudogene (2194/2434 nt) yqiG → putative outer membrane usher protein YqiG
RA 3,190,185 T→C L102P (CTT→CCT)  yqiH → putative fimbrial chaperone YqiH
RA 3,243,047 A→T Y90N (TAT→AAT)  uxaA ← D‑altronate dehydratase
RA 3,362,820 C→T T5M (ACG→ATG)  yhcD → putative fimbrial usher protein YhcD
RA 3,651,128 (T)7→8 intergenic (+466/‑163) arsC → / → yhiS arsenate reductase/putative uncharacterized protein YhiS
RA 3,667,617 (A)6→5 intergenic (‑37/+174) gadA ← / ← ccp glutamate decarboxylase A/cytochrome c peroxidase
RA 3,795,757 G→C A277P (GCG→CCG)  waaF → ADP‑heptose‑‑LPS heptosyltransferase 2
RA 3,797,159 (A)6→5 coding (212/1260 nt) waaL → O‑antigen ligase
RA 3,863,817 A→T intergenic (‑214/‑82) glvC ← / → yidP putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP
RA 3,941,650 A→G intergenic (‑323/‑158) yieP ← / → rrsC putative transcriptional regulator YieP/16S ribosomal RNA
RA 3,949,783 (T)7→6 coding (187/1521 nt) yifB ← putative magnesium chelatase YifB
RA 4,007,952 A→G K66E (AAA→GAA)  rhtC → L‑threonine exporter
RA 4,098,551 A→G G272G (GGA→GGG rhaS → DNA‑binding transcriptional activator RhaS
RA 4,109,886 T→C intergenic (+63/‑44) sbp → / → cdh sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp/CDP‑diacylglycerol diphosphatase
RA 4,175,853 (T)6→5 intergenic (+24/‑91) thrT → / → tufB tRNA‑Thr/translation elongation factor Tu 2
RA 4,371,109 G→A V29I (GTT→ATT)  groL → chaperonin GroEL
RA 4,503,704 A→G intergenic (‑273/‑354) insG ← / → yjhB KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter
RA 4,638,175 A→G intergenic (+55/‑3) creC → / → creD sensory histidine kinase CreC/putative inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 236049 236788 740 6 [5] [5] 7 [dnaQ] [dnaQ]
* * ÷ NC_000913 583430 583528 99 6 [5] [5] 6 tfaX/appY DLP12 prophage; protein TfaX/DNA‑binding transcriptional activator AppY
* * ÷ NC_000913 3423703–3424234 3424580–3424238 5–878 6 [5] [4] 6 [rrfD]–[rrlD] [rrfD],[rrlD]