Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,885,049 |
G→A |
P724L (CCT→CTT) |
cas3 ← |
CRISPR‑associated endonuclease/helicase Cas3 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,885,049 | 0 | G | A | 100.0%
| 65.4
/ NA
| 19 | P724L (CCT→CTT) | cas3 | CRISPR‑associated endonuclease/helicase Cas3 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/9); total (10/9) |
ATTGCCGACCCATTCTGGCTCATCCATTTCCGCATCATCGTAAATGCTATCCAGCCATTGCCGGTAAGCATCAGGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGACTCTAACGTTGCTATAAA > NC_000913/2884976‑2885124
|
aTTGCCGACCCATTCTGGCTCATCCATTTCCGCATCATCGTAAATGCTATCCAGCCATTGCCGGTAAGCATCAAGGaaaaa > 1:523392/1‑81 (MQ=255)
ttGCCGACCCATTCTGGCTCATCCATTTCCGCATCATCGTAAATGCTATCCAGCCATTGCCGGTAAGCATCAAGGAAAAAt < 1:523354/81‑1 (MQ=255)
cATTCTGGCTCATCCATTTCCGCATCATCGTAAATGCTATCCAGCCATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCt < 1:523355/81‑1 (MQ=255)
ttCTGGCTCATCCATTTCCGCATCATCGTAAATGCTATCCAGCCATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTcc < 1:523356/81‑1 (MQ=255)
aTCCATTTCCGCATCATCGTAAATGCTATCCAGCCATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGct < 1:523357/80‑1 (MQ=255)
ccGCATCATCGTAAATGCTATCCAGCCATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAatat > 1:523399/1‑81 (MQ=255)
tcatcGTAAATGCTATCCAGCCATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTtgc > 2:523394/1‑81 (MQ=255)
aaaTGCTATCCAGCCATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTcc > 2:523389/1‑81 (MQ=255)
aaaTGCTATCCAGCCATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTcc > 2:523395/1‑81 (MQ=255)
tATCCAGCCATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCcaca > 2:523387/1‑81 (MQ=255)
aGCCATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGAct > 2:523391/1‑81 (MQ=255)
ccATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGActct < 2:523358/81‑1 (MQ=255)
ccATTGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGActc > 2:523406/1‑80 (MQ=255)
ttGCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGACTCTAAc < 2:523359/81‑1 (MQ=255)
gCCGGTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGACTCTAACg < 2:523360/80‑1 (MQ=255)
gTAAGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGACTCTAACGTTGCt < 2:523361/81‑1 (MQ=255)
aaGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGACTCTAACGTTGCta > 2:523398/1‑80 (MQ=255)
aGCATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGACTCTAACGTTGCtat > 2:523408/1‑80 (MQ=255)
cATCAAGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGACTCTAACGTTGCTATaaa < 2:523362/81‑1 (MQ=255)
|
ATTGCCGACCCATTCTGGCTCATCCATTTCCGCATCATCGTAAATGCTATCCAGCCATTGCCGGTAAGCATCAGGGAAAAATAAGGATGCTCCATTAAGCTCCTCAATATGTTGCTGCGTCCGCCACATGACTCTAACGTTGCTATAAA > NC_000913/2884976‑2885124
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
