Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	730	35.1	2107516	91.4%	1926269	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,003,834	G→C	A116P (GCA→CCA)	xdhC →	putative xanthine dehydrogenase iron‑sulfur‑binding subunit XdhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,003,834	0	G	C	96.8%	103.0 / ‑3.4	31	A116P (GCA→CCA)	xdhC	putative xanthine dehydrogenase iron‑sulfur‑binding subunit XdhC
Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (14/16); total (14/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

ATGTTCAGCAGGCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGGCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGT  >  NC_000913/3003757‑3003908

aTGTTCAGCAGGCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCa                                                                          <  1:548258/80‑1 (MQ=255)

      agcagGCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGGTTATGGCTACCACGCCAATGCTgg                                                                   >  2:548283/1‑81 (MQ=255)

      agcagGCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGGCAATGCTg                                                                    <  1:548259/80‑1 (MQ=255)

      agcagGCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTgg                                                                   >  2:548299/1‑81 (MQ=255)

         agGCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGa                                                                <  2:548261/81‑1 (MQ=255)

         agGCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGa                                                                <  2:548260/81‑1 (MQ=255)

                tGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCAcg                                                         >  2:548317/1‑81 (MQ=255)

                   gAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCAcgc                                                        >  2:548310/1‑79 (MQ=255)

                    aaaTCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCAcgc                                                        >  1:548311/1‑78 (MQ=255)

                     aaTCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCgaga                                                    <  2:548262/81‑1 (MQ=255)

                      aTCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGaa                                                   <  2:548263/81‑1 (MQ=255)

                       tCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAg                                                  <  1:548264/81‑1 (MQ=255)

                         cGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGcc                                                <  2:548265/81‑1 (MQ=255)

                            cgcAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCAtt                                             <  2:548266/81‑1 (MQ=255)

                                   cagtgcGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCAt                                       <  2:548267/80‑1 (MQ=255)

                                     gtgcGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTAc                                    <  1:548268/81‑1 (MQ=255)

                                       gcGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACg                                   >  1:548314/1‑80 (MQ=255)

                                        cGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACgg                                  >  2:548326/1‑80 (MQ=255)

                                         gggTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGa                                 >  1:548304/1‑80 (MQ=255)

                                          ggTTTTGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGaaa                               <  1:548269/81‑1 (MQ=255)

                                            ttttGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAAtt                             <  1:548270/81‑1 (MQ=255)

                                            ttttGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAAtt                             >  2:548322/1‑81 (MQ=255)

                                              ttGTACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATtcg                           >  1:548298/1‑81 (MQ=255)

                                                 tACGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATtcgtc                         <  1:548271/80‑1 (MQ=255)

                                                  aCGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTcgc                       >  1:548329/1‑81 (MQ=255)

                                                  aCGCCTGGCCTGATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTcgc                       >  1:548293/1‑81 (MQ=255)

                                                          cctgATTATGGCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGc               <  1:548272/81‑1 (MQ=255)

                                                                   ggCTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCt      >  1:548327/1‑81 (MQ=255)

                                                                     cTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCttt    <  2:548273/81‑1 (MQ=255)

                                                                     cTACCACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCttt    >  2:548302/1‑81 (MQ=255)

                                                                        ccACGCCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGt  <  1:548274/80‑1 (MQ=255)

ATGTTCAGCAGGCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGGCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGT  >  NC_000913/3003757‑3003908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A