Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,064,741 |
T→C |
F250S (TTC→TCC) |
scpB → |
methylmalonyl‑CoA decarboxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,064,741 | 0 | T | C | 100.0%
| 135.7
/ NA
| 39 | F250S (TTC→TCC) | scpB | methylmalonyl‑CoA decarboxylase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/16); total (23/16) |
CCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAG > NC_000913/3064663‑3064813
|
ccGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTccc > 2:562690/1‑81 (MQ=255)
cGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTccc < 1:562646/80‑1 (MQ=255)
gATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTc > 2:562686/1‑81 (MQ=255)
gATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTc > 2:562694/1‑81 (MQ=255)
aTGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCg < 1:562647/81‑1 (MQ=255)
aaTTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGaa > 2:562692/1‑80 (MQ=255)
aaTTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGaa < 2:562648/80‑1 (MQ=255)
aTTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGaaaa < 1:562650/81‑1 (MQ=255)
aTTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGaaaa < 1:562649/81‑1 (MQ=255)
aTTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGaaa > 1:562681/1‑80 (MQ=255)
tttGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGaaaaa > 2:562709/1‑81 (MQ=255)
tGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAAc < 1:562651/80‑1 (MQ=255)
tGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACg > 2:562700/1‑81 (MQ=255)
ttCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCt > 1:562707/1‑80 (MQ=255)
aGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAAtt > 1:562695/1‑81 (MQ=255)
aGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAAtt > 1:562698/1‑81 (MQ=255)
ggATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCg < 2:562652/81‑1 (MQ=255)
aTGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGtt < 1:562653/81‑1 (MQ=255)
gcgcgGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCAt > 2:562684/1‑80 (MQ=255)
gcgGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTaa > 2:562703/1‑81 (MQ=255)
cgGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAAt > 2:562705/1‑81 (MQ=255)
cgGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAAt > 2:562710/1‑81 (MQ=255)
ggTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATc > 1:562687/1‑81 (MQ=255)
ggTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATc > 2:562715/1‑81 (MQ=255)
tgtATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATccc > 1:562704/1‑81 (MQ=255)
tATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCt < 1:562655/80‑1 (MQ=255)
tATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCt < 1:562654/80‑1 (MQ=255)
aCAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGcgaa > 2:562689/1‑81 (MQ=255)
cAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGcgaac < 2:562656/81‑1 (MQ=255)
cAGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGcgaac > 2:562706/1‑81 (MQ=255)
aGCGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGcgaac > 1:562711/1‑80 (MQ=255)
cGAAGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGcgaacgaa < 2:562657/81‑1 (MQ=255)
aaGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAgg < 1:562658/81‑1 (MQ=255)
aaGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAg > 2:562713/1‑80 (MQ=255)
aGATTACCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAg > 2:562725/1‑79 (MQ=255)
aCCAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAGGAGTaa > 2:562691/1‑80 (MQ=255)
cAGGAAGGGATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAGGAGTaaaaa < 1:562659/81‑1 (MQ=255)
aGGGATGAACGCTTCACTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAGGAGTAAAAATGGaa < 1:562660/81‑1 (MQ=255)
ggATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAGGAGTAAAAATGGAAAc < 1:562661/81‑1 (MQ=255)
gATGAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAGGAGTAAAAATGGAAACt < 1:562662/81‑1 (MQ=255)
gAACGCTTCCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAg > 1:562717/1‑81 (MQ=255)
|
CCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAATTTCGTTGGTCATTAATCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAG > NC_000913/3064663‑3064813
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
