Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,595,767 |
C→A |
A213S (GCG→TCG) |
livH ← |
branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,595,767 | 0 | C | A | 100.0%
| 113.9
/ NA
| 33 | A213S (GCG→TCG) | livH | branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/17); total (16/17) |
CGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCG > NC_000913/3595689‑3595843
|
cGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCgagc > 1:694719/1‑81 (MQ=255)
gCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGcc > 2:694713/1‑80 (MQ=255)
gCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGcc > 1:694710/1‑80 (MQ=255)
cGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAgg > 1:694728/1‑81 (MQ=255)
gTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAg > 2:694737/1‑81 (MQ=255)
tAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAgc < 1:694675/81‑1 (MQ=255)
aGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAgcg > 2:694733/1‑81 (MQ=255)
ggggTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAgcgc < 2:694676/81‑1 (MQ=255)
tGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCAccc < 1:694677/81‑1 (MQ=255)
gACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcg > 1:694723/1‑81 (MQ=255)
aCGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcgg > 2:694717/1‑81 (MQ=255)
gCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtc < 1:694678/81‑1 (MQ=255)
cGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtcgg > 2:694725/1‑81 (MQ=255)
aGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGgtgt > 1:694731/1‑81 (MQ=255)
gAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCGCAAAGGTCAGCGCAATCACCCGGTCGGTGtt < 1:694679/81‑1 (MQ=255)
aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATg < 1:694682/79‑1 (MQ=255)
aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGcc < 1:694681/81‑1 (MQ=255)
aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGc < 1:694680/80‑1 (MQ=255)
agcagcACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAg > 2:694730/1‑80 (MQ=255)
cagcaCACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAg > 2:694727/1‑78 (MQ=255)
cagcaCACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAg < 2:694683/81‑1 (MQ=255)
cacCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCg < 1:694684/81‑1 (MQ=255)
cGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCAt < 1:694685/81‑1 (MQ=255)
gCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCAtt > 1:694735/1‑81 (MQ=255)
ccACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCAttt < 1:694686/81‑1 (MQ=255)
aCCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCAtttt > 1:694745/1‑80 (MQ=255)
aCCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTc < 2:694687/81‑1 (MQ=255)
ccgccgCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCa < 1:694688/81‑1 (MQ=255)
gccgccATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCAtttt > 2:694736/1‑77 (MQ=255)
ccATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCtt > 2:694763/1‑81 (MQ=255)
ccATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCtt < 1:694689/81‑1 (MQ=255)
cATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTc > 2:694724/1‑81 (MQ=255)
aTCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTcc < 2:694691/81‑1 (MQ=255)
aTCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTcc < 1:694690/81‑1 (MQ=255)
gccgaGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCcgcg > 2:694739/1‑81 (MQ=255)
|
CGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCG > NC_000913/3595689‑3595843
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
