Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,486,291 |
T→A |
I25N (ATC→AAC) |
lptF → |
lipopolysaccharide transport system protein LptF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,486,291 | 0 | T | A | 100.0%
| 136.6
/ NA
| 38 | I25N (ATC→AAC) | lptF | lipopolysaccharide transport system protein LptF |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (21/17); total (21/17) |
GATAATCATAAGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGATCTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCC > NC_000913/4486220‑4486367
|
gATAATCATAAGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTg > 2:928883/1‑81 (MQ=255)
aTAATCATAAGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGt > 2:928858/1‑81 (MQ=255)
aTAATCATAAGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGt < 1:928822/81‑1 (MQ=255)
tAATCATAAGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTc < 2:928823/81‑1 (MQ=255)
aTCATAAGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCa < 2:928825/80‑1 (MQ=255)
aTCATAAGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCa < 2:928824/80‑1 (MQ=255)
tAAGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGt > 2:928863/1‑81 (MQ=255)
aGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGt > 1:928873/1‑79 (MQ=255)
tCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAg > 1:928861/1‑81 (MQ=255)
ggTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGAt > 2:928868/1‑81 (MQ=255)
ggTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGAt > 1:928877/1‑81 (MQ=255)
ggTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGAt > 1:928869/1‑81 (MQ=255)
gTGCGGGAGACGCT‑AAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATcc > 1:928890/1‑81 (MQ=255)
gCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCt > 2:928870/1‑81 (MQ=255)
gggAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCg > 2:928874/1‑81 (MQ=255)
ggAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCgg < 1:928826/81‑1 (MQ=255)
ggAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCgg < 1:928827/81‑1 (MQ=255)
aaaaGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGtt > 2:928904/1‑81 (MQ=255)
aaaaGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGtt < 1:928829/81‑1 (MQ=255)
aaaaGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGt < 2:928828/80‑1 (MQ=255)
aaGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGa > 2:928893/1‑81 (MQ=255)
aaGCCAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGa > 2:928884/1‑81 (MQ=255)
cAGCTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGc > 2:928876/1‑81 (MQ=255)
cTGGCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGat < 1:928830/81‑1 (MQ=255)
ggCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGatat > 1:928871/1‑81 (MQ=255)
gCGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGata < 2:928831/79‑1 (MQ=255)
cGATACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTc < 1:928832/81‑1 (MQ=255)
tACTCTTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCgg < 2:928833/81‑1 (MQ=255)
ctctTCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGAGCCTCGGCGCAGCGGTTGACGGCGATATTCCGGc < 1:928834/80‑1 (MQ=255)
ttCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAAt > 2:928879/1‑81 (MQ=255)
tCATCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATc > 2:928926/1‑81 (MQ=255)
aTCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTg > 2:928872/1‑81 (MQ=255)
aTCTTGCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTg > 2:928894/1‑81 (MQ=255)
gCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGt < 2:928835/78‑1 (MQ=255)
gCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTg < 1:928836/79‑1 (MQ=255)
gCTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGct > 1:928888/1‑81 (MQ=255)
cTTTTGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGctc < 2:928837/81‑1 (MQ=255)
tGAACTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTcc < 2:928838/80‑1 (MQ=255)
|
GATAATCATAAGATATCTGGTGCGGGAGACGCTCAAAAGCCAGCTGGCGATACTCTTCATCTTGCTTTTGATCTTCTTCTGTCAAAAGTTAGTGAGGATCCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCC > NC_000913/4486220‑4486367
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
