Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,983,085 |
(A)6→5 |
coding (174/762 nt) |
kduD ← |
putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,983,080 | 0 | A | . | 100.0%
| 133.4
/ NA
| 32 | coding (179/762 nt) | kduD | putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (21/11); total (21/11) |
AATATGACCAAACTCCGCTACCGCGCGATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACA > NC_000913/2983001‑2983156
|
aaTATGACCAAACTCCGCTACCGCGCGATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTaa < 2:488381/81‑1 (MQ=255)
tGACCAAACTCCGCTACCGCGCGATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTaaaaac < 1:488383/81‑2 (MQ=255)
cTCCGCTACCGCGCGATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGccc > 1:488410/1‑80 (MQ=255)
aCCGCGCGATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCtg > 2:488423/1‑81 (MQ=255)
ccGCGCGATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCtgt > 1:488413/1‑81 (MQ=255)
gcgcgATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGa > 1:488439/1‑81 (MQ=255)
gcgATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGAcc > 1:488414/1‑81 (MQ=255)
gcgATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGAcc > 1:488418/1‑81 (MQ=255)
gATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTg > 2:488430/1‑81 (MQ=255)
aTCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGc > 1:488406/1‑81 (MQ=255)
ccAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTc > 2:488416/1‑81 (MQ=255)
gcagTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGAt > 2:488415/1‑81 (MQ=255)
gcagTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGAt < 2:488383/81‑1 (MQ=255)
cagTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATg < 2:488384/81‑1 (MQ=255)
agTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATgg > 2:488434/1‑81 (MQ=255)
tGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGtt < 2:488385/81‑1 (MQ=255)
gCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGttt > 2:488427/1‑81 (MQ=255)
cTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTc < 1:488387/81‑1 (MQ=255)
aaTACCATCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTc > 1:488407/1‑81 (MQ=255)
aTCAATCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCa < 1:488388/81‑1 (MQ=255)
aaTCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAAc > 1:488420/1‑80 (MQ=255)
tCTTTCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGAt > 2:488429/1‑81 (MQ=255)
ttCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGt > 2:488426/1‑80 (MQ=255)
tCGCAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGAt < 2:488388/77‑1 (MQ=255)
cAGATCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATg > 1:488422/1‑81 (MQ=255)
aTCGGCGGTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGcc > 1:488421/1‑80 (MQ=255)
ggcggTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCcaac < 2:488389/80‑1 (MQ=255)
ggcggTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCcaac > 2:488425/1‑80 (MQ=255)
cggTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAAt > 2:488432/1‑81 (MQ=255)
gTCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGt < 1:725804/81‑1 (MQ=255)
tCAGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTc < 1:488391/81‑1 (MQ=255)
aGGCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTcac < 1:488392/81‑1 (MQ=255)
ggCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTcaca < 2:488392/81‑1 (MQ=255)
ggCTT‑AAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTca > 1:488423/1‑79 (MQ=255)
|
AATATGACCAAACTCCGCTACCGCGCGATCCAGCAGTGCTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACA > NC_000913/2983001‑2983156
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
