Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,989,072 |
G→A |
E191K (GAA→AAA) |
yqeI → |
putative transcriptional regulator YqeI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,989,072 | 0 | G | A | 100.0%
| 74.0
/ NA
| 21 | E191K (GAA→AAA) | yqeI | putative transcriptional regulator YqeI |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/11); total (10/11) |
AGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAAGAGTTCGATAAATATA > NC_000913/2988996‑2989141
|
aGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAAt > 2:489420/1‑81 (MQ=255)
ggCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAAtt < 1:489404/81‑1 (MQ=255)
ggCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAAt > 2:489434/1‑80 (MQ=255)
cTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGc < 1:489405/80‑1 (MQ=255)
tGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAAt > 1:489423/1‑81 (MQ=255)
gCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGt < 2:489405/81‑1 (MQ=255)
aTGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTaa > 1:489428/1‑80 (MQ=255)
aTGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTaa > 2:489425/1‑80 (MQ=255)
tGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAAc < 1:489407/80‑1 (MQ=255)
ataataatCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACag > 1:489427/1‑78 (MQ=255)
ataatCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATa < 2:489407/81‑1 (MQ=255)
tCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAAt < 1:489409/81‑1 (MQ=255)
gCCATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGtt < 2:489409/81‑1 (MQ=255)
cATTTTTCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTg > 2:489424/1‑80 (MQ=255)
tttCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAag < 1:489411/80‑1 (MQ=255)
tCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAagag < 2:489411/80‑1 (MQ=255)
tCAAGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAAGAGt > 1:489431/1‑81 (MQ=255)
aaGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAAGAGtt < 1:489413/80‑1 (MQ=255)
aaGGATTACAAGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAAGAGTTc > 1:489429/1‑81 (MQ=255)
aaGACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAAGAGTTCGATAAatat < 1:489414/81‑1 (MQ=255)
gACTGTTGCGAAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAAGAGTTCGATAAAtata > 1:489434/1‑80 (MQ=255)
|
AGGCGCATATTCTGCATATTGGCTATGGAATAATAATCAACCGAAGCCATTTTTCAAGGATTACAAGACTGTTGCGGAAATTAATGGCTGCCATTTTAATGTAACAGAAGATACAATTGATGGGTTGAAAGAGTTCGATAAATATA > NC_000913/2988996‑2989141
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
