Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,057,133 |
(A)7→8 |
intergenic (+142/+45) |
sibC → / ← serA |
small RNA SibC/phosphoglycerate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,057,126 | 1 | . | A | 100.0%
| 62.9
/ NA
| 18 | intergenic (+135/+52) | sibC/serA | small RNA SibC/phosphoglycerate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (9/9); total (9/9) |
CTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAAT > NC_000913/3057053‑3057201
|
cTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑aaaaaa > 1:503121/1‑80 (MQ=255)
gACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑aaaaaaaac > 2:503116/1‑79 (MQ=255)
aCTTTGATTTATTTTCTGGAACGGACTCGAAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑aaaaa < 1:503087/76‑1 (MQ=255)
cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑aaaaaaaacgg > 2:503111/1‑77 (MQ=255)
tGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGc < 2:590395/80‑1 (MQ=255)
ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGt < 1:503089/81‑1 (MQ=255)
tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGt > 1:503119/1‑80 (MQ=255)
gAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGAcc < 2:503089/78‑1 (MQ=255)
gAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCt < 1:503091/79‑1 (MQ=255)
cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCg > 1:503126/1‑81 (MQ=255)
aGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGt < 1:503092/81‑1 (MQ=255)
gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGtt < 1:503093/81‑1 (MQ=255)
tCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt > 1:503123/1‑81 (MQ=255)
aaaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtttt > 1:503122/1‑80 (MQ=255)
aGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag > 2:503127/1‑80 (MQ=255)
aaCGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt < 1:503094/81‑1 (MQ=255)
aaCGCGGCAACGGTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAt > 1:503134/1‑80 (MQ=255)
ggTGTGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGa > 2:503136/1‑81 (MQ=255)
gtgtGGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGAc > 2:503124/1‑81 (MQ=255)
tgGAGAAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACggg > 2:503126/1‑81 (MQ=255)
gagaAGGGATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg < 1:503095/81‑1 (MQ=255)
gggATAAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAAt < 1:503096/81‑1 (MQ=255)
|
CTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGAT‑AAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAAT > NC_000913/3057053‑3057201
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
