Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,429,412 |
T→C |
N59N (AAT→AAC) |
yrdA → |
protein YrdA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,429,412 | 0 | T | C | 100.0%
| 105.4
/ NA
| 31 | N59N (AAT→AAC) | yrdA | protein YrdA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/12); total (19/12) |
GGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATT > NC_000913/3429334‑3429477
|
ggCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAacat > 2:586892/1‑81 (MQ=255)
tgatgaTGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATc > 1:586890/1‑79 (MQ=255)
atgatgTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAgg < 1:586843/81‑1 (MQ=255)
atgTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATg > 2:586875/1‑81 (MQ=255)
atgTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATg > 1:586871/1‑81 (MQ=255)
gtgGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATgg > 1:586884/1‑80 (MQ=255)
tgGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGc < 1:586844/80‑1 (MQ=255)
ggATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTa < 1:586845/81‑1 (MQ=255)
cTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGtt > 2:586886/1‑81 (MQ=255)
cTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGtt > 2:586874/1‑81 (MQ=255)
gCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGt < 1:586846/77‑1 (MQ=255)
tCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCa > 2:586882/1‑81 (MQ=255)
gTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACt > 2:586879/1‑81 (MQ=255)
gTTGTGATTCGTGGAGATGTACATTATGTACAGATCGAAGCACGCACCAACATCCAGGATGACAGTATGTTGCATGTCa < 2:586846/79‑1 (MQ=255)
tgATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCAt > 2:586899/1‑80 (MQ=255)
tgATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATa < 1:586848/81‑1 (MQ=255)
cGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATcc > 2:586894/1‑81 (MQ=255)
tGGAGATGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTc < 2:586848/81‑1 (MQ=255)
gagaTGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCg > 2:586895/1‑80 (MQ=255)
agaTGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTa < 1:586850/81‑1 (MQ=255)
aTGTACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTAc > 1:586898/1‑80 (MQ=255)
tACATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAc < 2:586850/80‑1 (MQ=255)
aCATTATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAAcc > 2:586884/1‑80 (MQ=255)
ttATGTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGa < 1:586852/81‑1 (MQ=255)
gTACAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATgg > 1:586909/1‑80 (MQ=255)
cAGATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAAc > 2:586893/1‑81 (MQ=255)
gATCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAAcc > 1:586899/1‑80 (MQ=255)
gATCGGAGCACGCACCAACATCCAGGATGACAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAAccc < 1:586853/81‑1 (MQ=255)
aTCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCa > 1:586901/1‑81 (MQ=255)
tCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAt < 1:586854/81‑1 (MQ=255)
tCGGAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAt > 2:586904/1‑81 (MQ=255)
ggAGCACGCACCAACATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCagt > 2:586907/1‑78 (MQ=255)
|
GGCTGATGATGTGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATT > NC_000913/3429334‑3429477
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
