Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,595,737 |
G→T |
L223I (CTC→ATC) |
livH ← |
branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,595,737 | 0 | G | T | 100.0%
| 167.5
/ NA
| 47 | L223I (CTC→ATC) | livH | branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (26/21); total (26/21) |
CGAGCACCGCCGCGGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAA > NC_000913/3595659‑3595814
|
cGAGCACCGCCGCGGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGatca < 1:628171/81‑1 (MQ=255)
gCACCGCCGCGGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAtcagca > 1:628229/1‑81 (MQ=255)
cACCGCCGCGGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAtcagca > 1:628233/1‑80 (MQ=255)
aCCGCCGCGGTAAAGGCTTTCATCCCGGCCATAAGGCCGATGTAGGGGTTAATGACGCCGTAGAACTGCCCGATCAGcaca < 2:628171/81‑1 (MQ=255)
gccgcGGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACAccc > 2:628254/1‑81 (MQ=255)
ccgcGGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCg < 2:628172/81‑1 (MQ=255)
cgcgGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGc > 2:628243/1‑81 (MQ=255)
cgcgGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGc > 1:628221/1‑81 (MQ=255)
gcgGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGcc < 2:628173/81‑1 (MQ=255)
cgGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCa < 1:628176/81‑1 (MQ=255)
cgGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCa < 2:628174/81‑1 (MQ=255)
cgGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCa > 1:628228/1‑81 (MQ=255)
ggTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCAc > 2:628219/1‑81 (MQ=255)
aaGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCAccgcc > 1:628227/1‑81 (MQ=255)
aaGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCAccgcc > 2:628222/1‑81 (MQ=255)
ggCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACcgccgc > 2:628245/1‑81 (MQ=255)
tttCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCAt > 2:628235/1‑81 (MQ=255)
tCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATc < 1:628177/80‑1 (MQ=255)
aTCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATcgcc > 2:628251/1‑81 (MQ=255)
aTCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATcgc < 2:628178/80‑1 (MQ=255)
aTCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATcgc < 2:628177/80‑1 (MQ=255)
tCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATcgccg < 1:628180/81‑1 (MQ=255)
ccGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATcgccga < 2:628180/80‑2 (MQ=255)
ggCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGcc < 1:628182/81‑1 (MQ=255)
cATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAAt > 1:628231/1‑81 (MQ=255)
aTAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATc < 2:628182/81‑1 (MQ=255)
aaaGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATca > 2:628229/1‑80 (MQ=255)
aTGTAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTc < 2:628183/81‑1 (MQ=255)
tAGGGGTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAgc > 1:628237/1‑81 (MQ=255)
ggggTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAgcgc < 2:628184/81‑1 (MQ=255)
ggggTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAgcgc < 2:628185/81‑1 (MQ=255)
gTTGATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAAt > 2:628237/1‑81 (MQ=255)
gTTAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAAt > 1:628235/1‑81 (MQ=255)
ttAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAAt > 1:628242/1‑80 (MQ=255)
ttAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATc < 1:628187/81‑1 (MQ=255)
tAATGACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCa > 2:628256/1‑81 (MQ=255)
gACGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcg > 1:628256/1‑81 (MQ=255)
cGCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggt > 2:628253/1‑81 (MQ=255)
gCCGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtc > 1:628250/1‑81 (MQ=255)
ccGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtcg < 2:628187/81‑1 (MQ=255)
cGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtcgg > 1:628239/1‑81 (MQ=255)
cGTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtcgg > 1:628243/1‑81 (MQ=255)
gTAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCcggtcggt > 1:628234/1‑81 (MQ=255)
tAGAACTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGTGCAATCACCCGGTCGgtg < 1:628189/81‑1 (MQ=255)
aaCTGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTa < 2:628189/81‑1 (MQ=255)
tGACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATg > 1:628240/1‑81 (MQ=255)
gACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGc > 2:628250/1‑81 (MQ=255)
gACCGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGc > 2:628240/1‑81 (MQ=255)
ccGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCa < 1:628191/81‑1 (MQ=255)
cGATCAGCACACCCGCCACCGCCGCCATCGCCGAGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCaa < 1:628192/81‑1 (MQ=255)
|
CGAGCACCGCCGCGGTAAAGGCTTTCATCCCGGCCATAAAGCCGATGTAGGGGTTAATGACGCCGTAGAACTGACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAA > NC_000913/3595659‑3595814
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
