Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,605,159 |
C→T |
Q61* (CAG→TAG) |
yhhL → |
DUF1145 domain‑containing protein YhhL |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,605,159 | 0 | C | T | 100.0%
| 114.7
/ NA
| 32 | Q61* (CAG→TAG) | yhhL | DUF1145 domain‑containing protein YhhL |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/17); total (15/17) |
GTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGAAGAAATTTAA > NC_000913/3605081‑3605235
|
gTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAg < 1:630526/81‑1 (MQ=255)
cTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGac > 1:630577/1‑80 (MQ=255)
aTTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGaccacc < 1:630527/81‑1 (MQ=255)
tttACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCAccgcc > 1:630584/1‑81 (MQ=255)
ttACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCAccgcc > 1:630565/1‑80 (MQ=255)
aCCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGa > 2:630579/1‑80 (MQ=255)
gTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAg > 2:630582/1‑81 (MQ=255)
aTGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACg > 2:630575/1‑80 (MQ=255)
tGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGa < 1:630528/81‑1 (MQ=255)
cATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGAtt < 1:630529/81‑1 (MQ=255)
aTGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGAttt > 1:630568/1‑81 (MQ=255)
tGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGAtttt < 2:630529/81‑1 (MQ=255)
tGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGAtttt < 2:630530/81‑1 (MQ=255)
gTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTcc > 2:630593/1‑81 (MQ=255)
tATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCt < 2:630531/81‑1 (MQ=255)
gcTATTGAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGtt > 1:630578/1‑81 (MQ=255)
gAAATCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAAc < 1:630533/80‑1 (MQ=255)
aaaTCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActg > 1:630588/1‑81 (MQ=255)
aaaTCCACTTTACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAAct < 1:630534/80‑1 (MQ=255)
tttACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctg > 1:630586/1‑81 (MQ=255)
tttACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctg > 1:630579/1‑81 (MQ=255)
ttACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctgg > 2:630590/1‑81 (MQ=255)
tACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctgg < 2:630535/80‑1 (MQ=255)
tACCGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctg < 1:630535/79‑1 (MQ=255)
ccGAAAGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCa < 1:630537/80‑1 (MQ=255)
aaaGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCagaag < 2:630537/81‑1 (MQ=255)
aGATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAgaagaa < 2:630538/81‑1 (MQ=255)
gATGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGAAGaaa > 1:630575/1‑81 (MQ=255)
tGGCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGAAGAAAtt < 2:630539/81‑1 (MQ=255)
ggCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGAAGAAAtt > 2:630600/1‑80 (MQ=255)
gCCCGTAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGAAGAAAttt < 1:630541/80‑1 (MQ=255)
cccGTAGATGACCACCGCCGAAAAGGTACGGGTTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGAAGAAATTTaa < 2:630541/81‑1 (MQ=255)
|
GTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGAAGAAATTTAA > NC_000913/3605081‑3605235
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
