Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,478,540 |
A→C |
E23A (GAA→GCA) |
rraB → |
ribonuclease E inhibitor protein B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,478,540 | 0 | A | C | 100.0%
| 143.4
/ NA
| 42 | E23A (GAA→GCA) | rraB | ribonuclease E inhibitor protein B |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (24/18); total (24/18) |
AATGAGCCATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGAAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAACCCTGGAAAAAG > NC_000913/4478465‑4478617
|
aaTGAGCCATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATg > 2:857056/1‑81 (MQ=255)
aTGAGCCATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATgg > 1:857055/1‑81 (MQ=255)
aTGAGCCATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATgg < 2:857011/81‑1 (MQ=255)
tGAGCCATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGc < 2:857012/81‑1 (MQ=255)
aGCCATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCa < 2:857013/80‑1 (MQ=255)
gCCATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGc > 2:857043/1‑81 (MQ=255)
gCCATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGc < 1:857015/81‑1 (MQ=255)
aTGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGAt > 2:857045/1‑81 (MQ=255)
ggCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGAAcc > 2:857053/1‑81 (MQ=255)
aaaCCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGa > 2:857052/1‑81 (MQ=255)
ccGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGAcgcg > 2:857060/1‑81 (MQ=255)
gAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATCACTGGCAGATGGCAGCGATCCGGACGCGCTg > 2:857066/1‑81 (MQ=255)
aacaacTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTg > 1:857052/1‑80 (MQ=255)
aacaacTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGt < 1:857016/81‑1 (MQ=255)
acaacTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTa > 2:857040/1‑81 (MQ=255)
caacTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTa > 2:857048/1‑80 (MQ=255)
gaagaaCAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATc < 2:857017/81‑1 (MQ=255)
gaagaaCAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATc < 1:857017/81‑1 (MQ=255)
aGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAacac > 1:857073/1‑80 (MQ=255)
gCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCa < 1:857019/81‑1 (MQ=255)
gTGAAGAAACACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATc < 2:857019/81‑1 (MQ=255)
aaaCACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCg > 2:857073/1‑81 (MQ=255)
aaCACGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCg > 1:857069/1‑80 (MQ=255)
acacGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCg > 1:857098/1‑79 (MQ=255)
acGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGa > 1:857081/1‑81 (MQ=255)
cGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGAc < 2:857020/81‑1 (MQ=255)
cGTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGAc < 2:857021/81‑1 (MQ=255)
gTTTGATTATTGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACg < 2:857022/81‑1 (MQ=255)
tattGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTgg > 2:857050/1‑80 (MQ=255)
tattGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTgg > 1:857065/1‑80 (MQ=255)
attGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGa < 2:857023/80‑1 (MQ=255)
attGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGa < 1:857026/80‑1 (MQ=255)
attGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGa < 2:857024/80‑1 (MQ=255)
tGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAAc > 1:857068/1‑81 (MQ=255)
tGAAGAATTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAAc > 2:857084/1‑81 (MQ=255)
aagaaTTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAAccc > 1:857062/1‑81 (MQ=255)
aagaaTTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAAcc < 1:857027/80‑1 (MQ=255)
agaaTTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAACCCt < 1:857028/81‑1 (MQ=255)
gaaTTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAACCCTg < 2:857028/81‑1 (MQ=255)
aaTTACTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAACCCTg > 1:857075/1‑80 (MQ=255)
aCTGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAACCCTGGaaa > 2:857065/1‑80 (MQ=255)
tGGCAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAACCCTGGAAAAag > 1:857070/1‑81 (MQ=255)
|
AATGAGCCATGGCAAACCCGGAACAACTGGAAGAACAGCGTGAAGAAACACGTTTGATTATTGAAGAATTACTGGAAGATGGCAGCGATCCGGACGCGCTGTACACCATCGAACACCATCTTTCCGCAGACGATCTGGAAACCCTGGAAAAAG > NC_000913/4478465‑4478617
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
