Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,504,957 |
(T)8→7 |
coding (900/1218 nt) |
yjhB → |
putative sialic acid transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,504,950 | 0 | T | . | 100.0%
| 81.3
/ NA
| 20 | coding (893/1218 nt) | yjhB | putative sialic acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (15/5); total (15/5) |
GATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATT > NC_000913/4504883‑4505026
|
gATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCtg > 1:862945/1‑81 (MQ=255)
aGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGa > 1:862933/1‑80 (MQ=255)
aTTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGaaa > 2:862935/1‑80 (MQ=255)
aTTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTGCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGaaa > 2:862934/1‑80 (MQ=255)
aaaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAActcttctct > 1:862949/1‑80 (MQ=255)
aaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTta > 1:862943/1‑81 (MQ=255)
ttGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGAt > 1:862950/1‑80 (MQ=255)
aGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGt > 2:862953/1‑81 (MQ=255)
ggTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtctc < 2:862917/80‑1 (MQ=255)
gTCTAATAACTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCtt < 1:862919/81‑1 (MQ=255)
aataaCTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGa > 2:862952/1‑81 (MQ=255)
ataaCTTCATTTATTTTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAt > 2:862938/1‑81 (MQ=255)
tttatttTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTa < 2:862919/81‑1 (MQ=255)
tttatttTCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGACTAATGTTTa > 2:862945/1‑81 (MQ=255)
tCCTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAtt > 2:862961/1‑80 (MQ=255)
cTTTGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAg > 1:862951/1‑81 (MQ=255)
tGTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAgg < 2:862920/79‑1 (MQ=255)
gTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAt > 1:862952/1‑81 (MQ=255)
gTCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAt > 2:862957/1‑81 (MQ=255)
tCCTC‑TTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt < 2:862921/81‑1 (MQ=255)
|
GATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATT > NC_000913/4504883‑4505026
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
