Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,231,953 |
C→T |
R256R (CGC→CGT) |
umuC → |
DNA polymerase V catalytic protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,231,953 | 0 | C | T | 100.0%
| 80.3
/ NA
| 24 | R256R (CGC→CGT) | umuC | DNA polymerase V catalytic protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/12); total (12/12) |
CGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCG > NC_000913/1231878‑1232026
|
cgAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCAc > 1:221527/1‑81 (MQ=255)
cccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGAt > 2:221531/1‑81 (MQ=255)
tttGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATcc < 1:221496/81‑1 (MQ=255)
ggAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCg > 2:221533/1‑81 (MQ=255)
ggAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCg > 1:221514/1‑81 (MQ=255)
gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGc < 2:221497/79‑1 (MQ=255)
gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCgg > 2:221538/1‑81 (MQ=255)
gCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCAt < 2:221498/80‑1 (MQ=255)
gacgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAg > 1:221523/1‑81 (MQ=255)
cgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGtt < 1:221499/81‑1 (MQ=255)
gCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGc > 2:221539/1‑81 (MQ=255)
gCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGc > 2:221530/1‑81 (MQ=255)
aTCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggc < 1:221500/80‑1 (MQ=255)
tCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggcgg > 2:221534/1‑81 (MQ=255)
tCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggcg < 2:221501/80‑1 (MQ=255)
tCTGTTCCCGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggcg < 1:221502/80‑1 (MQ=255)
agtacccGCTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGgcggcg < 2:221503/77‑1 (MQ=255)
cTCGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACt < 1:221504/81‑1 (MQ=255)
cGTTTGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTc > 2:221543/1‑81 (MQ=255)
ttGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCg > 1:221536/1‑79 (MQ=255)
tGGTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCa < 2:221505/80‑1 (MQ=255)
ggTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGc > 2:221522/1‑81 (MQ=255)
gTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGTAAAAACTTCGCAGc < 2:221507/80‑1 (MQ=255)
gTGAACGTATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCg < 2:221506/81‑1 (MQ=255)
|
CGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCG > NC_000913/1231878‑1232026
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
