Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,238,385 |
T→G |
L272R (CTG→CGG) |
dadA → |
D‑amino acid dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,238,385 | 0 | T | G | 100.0%
| 64.3
/ NA
| 19 | L272R (CTG→CGG) | dadA | D‑amino acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (8/11); total (8/11) |
GATGCGTATGTGATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCTGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATCCACCATTCTTGATGAAACC > NC_000913/1238309‑1238458
|
gATGCGTATGTGATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGa > 2:241566/1‑79 (MQ=255)
aTGTGATGGCGATTGGTTCTTACTCGACGGCGATGCCCAAAGGCATTGCTCATAATTAGGTGTATCCGCGGAAAGGCTACt > 2:241604/1‑81 (MQ=255)
tgtgATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTc < 1:241527/81‑1 (MQ=255)
tgtgATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTc < 2:241528/81‑1 (MQ=255)
tgATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGc < 2:241529/81‑1 (MQ=255)
aTGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTg < 1:241530/81‑1 (MQ=255)
tGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGa < 2:241531/81‑1 (MQ=255)
tttGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCAtt < 2:241532/81‑1 (MQ=255)
ggTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTcc < 1:241533/80‑1 (MQ=255)
cTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAAtt < 1:241534/80‑1 (MQ=255)
aCTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTgcg > 1:241560/1‑80 (MQ=255)
tCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAg > 1:241559/1‑81 (MQ=255)
gACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAgg < 1:241535/80‑1 (MQ=255)
cGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATg > 2:241568/1‑81 (MQ=255)
aaaGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGcc < 2:241536/80‑1 (MQ=255)
aGGCATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCgg > 2:241563/1‑80 (MQ=255)
cATTGTTGATATTCCGGTGTATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATc > 2:241561/1‑81 (MQ=255)
tATCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATCCACCATTCTTGATGAAAcc < 1:241537/81‑1 (MQ=255)
aTCCGCGGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATCCACCATTCTTGATGAAAcc > 2:241572/1‑80 (MQ=255)
|
GATGCGTATGTGATGGCGTTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATATTCCGGTGTATCCGCTGAAAGGCTACTCGCTGACCATTCCAATTGCGCAGGAAGATGGTGCGCCGGTATCCACCATTCTTGATGAAACC > NC_000913/1238309‑1238458
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
