Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,261,459 |
G→A |
F139F (TTC→TTT) |
prs ← |
ribose‑phosphate diphosphokinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,261,459 | 0 | G | A | 100.0%
| 96.5
/ NA
| 28 | F139F (TTC→TTT) | prs | ribose‑phosphate diphosphokinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/16); total (12/16) |
GGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTG > NC_000913/1261385‑1261535
|
gggTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGaaa > 1:226362/1‑81 (MQ=255)
ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGaa > 2:226351/1‑81 (MQ=255)
ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGaa > 1:226352/1‑81 (MQ=255)
gATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCt > 2:226359/1‑81 (MQ=255)
aGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTc < 2:226316/80‑1 (MQ=255)
gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAg < 1:226317/81‑1 (MQ=255)
cTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGc > 2:226365/1‑81 (MQ=255)
cagcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTg < 2:226318/81‑1 (MQ=255)
tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGAt < 2:226319/81‑1 (MQ=255)
tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATc < 2:226320/80‑1 (MQ=255)
cagcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGt > 1:226366/1‑81 (MQ=255)
agcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTc < 1:226321/81‑1 (MQ=255)
ggATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGca > 1:226370/1‑81 (MQ=255)
gATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGcac < 2:226322/81‑1 (MQ=255)
tCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCacac < 1:226323/81‑1 (MQ=255)
cGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACg > 1:226369/1‑81 (MQ=255)
cGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACg > 2:226364/1‑81 (MQ=255)
gggCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACgg > 1:226368/1‑81 (MQ=255)
cTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCa < 1:226324/81‑1 (MQ=255)
ccAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAac < 2:226325/80‑1 (MQ=255)
cGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGc > 2:226361/1‑81 (MQ=255)
aTCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTgg < 1:226327/80‑1 (MQ=255)
aTCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTgg < 2:226326/80‑1 (MQ=255)
aCCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg < 2:226328/81‑1 (MQ=255)
cGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg > 1:226377/1‑79 (MQ=255)
gAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGt < 1:226329/81‑1 (MQ=255)
aaCGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTc < 1:226330/81‑1 (MQ=255)
cGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTg < 2:226331/81‑1 (MQ=255)
|
GGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTG > NC_000913/1261385‑1261535
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
