Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,290,291 |
A→C |
Q17P (CAG→CCG) |
rssB → |
regulator of RpoS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,290,291 | 0 | A | C | 100.0%
| 74.6
/ NA
| 22 | Q17P (CAG→CCG) | rssB | regulator of RpoS |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (17/5); total (17/5) |
AGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT > NC_000913/1290217‑1290367
|
aGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTAtt > 2:231210/1‑81 (MQ=255)
gTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTAttt < 2:231192/81‑1 (MQ=255)
aaaGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTtcg > 2:231213/1‑81 (MQ=255)
cAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTtcgctcgc > 1:231214/1‑81 (MQ=255)
aGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTcgctcgct < 2:231193/81‑1 (MQ=255)
aGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCt < 1:231194/80‑1 (MQ=255)
aTGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGttt > 1:231208/1‑81 (MQ=255)
gACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTc > 2:231227/1‑81 (MQ=255)
gCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCAtt < 1:231195/81‑1 (MQ=255)
tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAg > 2:231224/1‑81 (MQ=255)
ggTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGc > 1:231218/1‑81 (MQ=255)
gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGc > 2:231220/1‑80 (MQ=255)
cGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGa > 1:231226/1‑80 (MQ=255)
ggAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGAc > 1:231222/1‑80 (MQ=255)
aCAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACgg > 1:231223/1‑80 (MQ=255)
gttgAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt > 2:231225/1‑81 (MQ=255)
tgAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg > 1:231228/1‑81 (MQ=255)
gAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATggg > 1:231233/1‑81 (MQ=255)
aGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt > 2:231221/1‑81 (MQ=255)
aGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt < 1:231196/81‑1 (MQ=255)
gATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTg > 1:231232/1‑81 (MQ=255)
gAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAt > 1:231235/1‑81 (MQ=255)
|
AGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT > NC_000913/1290217‑1290367
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
