Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,301,472 |
C→T |
V97V (GTC→GTT) |
oppA → |
oligopeptide ABC transporter periplasmic binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,301,472 | 0 | C | T | 100.0%
| 117.3
/ NA
| 34 | V97V (GTC→GTT) | oppA | oligopeptide ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/20); total (14/20) |
CTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTCTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCTG > NC_000913/1301401‑1301549
|
cTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTc < 2:232757/81‑1 (MQ=255)
tGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTc > 2:232791/1‑80 (MQ=255)
gTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCAt < 1:232758/81‑1 (MQ=255)
gCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGc > 2:232800/1‑81 (MQ=255)
gATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCg > 2:232793/1‑80 (MQ=255)
gACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTaaa < 2:232760/78‑1 (MQ=255)
gACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGa < 2:232759/80‑1 (MQ=255)
aCGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATg > 2:232804/1‑81 (MQ=255)
cGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGc < 2:232761/81‑1 (MQ=255)
ggTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCg < 2:232762/81‑1 (MQ=255)
cATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGaaa > 2:232802/1‑81 (MQ=255)
cAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGt < 2:232763/81‑1 (MQ=255)
aGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTc > 1:232816/1‑81 (MQ=255)
aGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTc > 2:232810/1‑81 (MQ=255)
gCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCt < 1:232765/81‑1 (MQ=255)
gCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCt < 1:232764/81‑1 (MQ=255)
gCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGc > 2:232801/1‑79 (MQ=255)
gCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGc > 2:232803/1‑79 (MQ=255)
aaTCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTc < 2:232766/80‑1 (MQ=255)
aaTCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTc < 2:232767/80‑1 (MQ=255)
aTCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTcac < 1:232768/81‑1 (MQ=255)
aTCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTca > 1:232821/1‑80 (MQ=255)
ggATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGcac > 2:232809/1‑80 (MQ=255)
ggATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGca < 2:232769/79‑1 (MQ=255)
ataataAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAg < 2:232770/81‑1 (MQ=255)
taataaAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGa > 2:232812/1‑81 (MQ=255)
taaAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACtt > 1:232806/1‑81 (MQ=255)
aaGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACttt < 1:232772/80‑1 (MQ=255)
aaGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACttt < 1:232771/80‑1 (MQ=255)
aCGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTgtgt < 2:232773/81‑1 (MQ=255)
aCGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTgtgt < 1:232774/81‑1 (MQ=255)
cgcgAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGta < 2:232775/81‑1 (MQ=255)
gAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAg > 1:232813/1‑81 (MQ=255)
aGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCTg < 2:232776/81‑1 (MQ=255)
|
CTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTCTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCTG > NC_000913/1301401‑1301549
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
