Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,306,654 |
T→C |
G297G (GGT→GGC) |
oppF → |
murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,306,654 | 0 | T | C | 100.0%
| 61.5
/ NA
| 18 | G297G (GGT→GGC) | oppF | murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/12); total (6/12) |
ATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAG > NC_000913/1306578‑1306725
|
aTCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGCtg < 2:233556/79‑1 (MQ=255)
aTCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCC‑CCTTCCGGCtgtg < 2:233555/80‑1 (MQ=255)
gATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTc > 1:233586/1‑81 (MQ=255)
aaCAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGc < 2:233557/81‑1 (MQ=255)
aCAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGcc < 2:233558/81‑1 (MQ=255)
ccATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTg > 1:233584/1‑81 (MQ=255)
ccAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCgg > 2:233578/1‑81 (MQ=255)
gTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTc > 1:233587/1‑80 (MQ=255)
gAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGt < 2:233559/79‑1 (MQ=255)
aaGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTgcg < 1:233560/81‑1 (MQ=255)
ggAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCaa < 2:233561/81‑1 (MQ=255)
gAATTACCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCaa > 2:233590/1‑80 (MQ=255)
aCCGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAacac < 2:233562/80‑1 (MQ=255)
cGTCGCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTc < 2:233563/81‑1 (MQ=255)
gCCGATCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTg < 2:233564/80‑1 (MQ=255)
aTCAACCCGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTg < 2:233565/81‑1 (MQ=255)
ccGCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAgggg < 1:233566/81‑1 (MQ=255)
gCCTTCCGGCTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAg > 1:233567/1‑81 (MQ=255)
|
ATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAG > NC_000913/1306578‑1306725
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
